Extracellular release of antigenic proteins by Helicobacter pylori

• Published in: Infection and immunity Vol. 66; no. 6; pp. 2984 - 2986
• Main Authors: PING CAO, MCCLAIN, M. S, FORSYTH, M. H, COVER, T. L
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.06.1998
• Series: Note
• Subjects: Amino Acid Sequence; Antigens, Bacterial - genetics; Antigens, Bacterial - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Biological and medical sciences; Escherichia coli Proteins; Fundamental and applied biological sciences. Psychology; Genomic Library; Heat-Shock Proteins - genetics; Heat-Shock Proteins - metabolism; Helicobacter pylori - genetics; Helicobacter pylori - immunology; Helicobacter pylori - metabolism; Hemolysin Proteins; HSP70 Heat-Shock Proteins - genetics; HSP70 Heat-Shock Proteins - metabolism; Lipoproteins - genetics; Lipoproteins - metabolism; Membrane Transport Proteins; Microbiology; Molecular and Cellular Pathogenesis; Molecular Sequence Data; Permeability, membrane transport, intracellular transport; Plasmids - genetics; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Antigen; Characterization; Spirillales; Helicobacter pylori; Spirillaceae; Bacteria; Extracellular; Release; Protein
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/iai.66.6.2984-2986.1998

Screening a Helicobacter pylori genomic library with antisera raised against H. pylori broth culture supernatant resulted in the identification of six antigens: urease, HspB, Lpp20, DnaK, MsrA, and a cysteine-rich 28-kDa protein (designated HcpA). H. pylori antigens may be released into the extracellular space by multiple mechanisms, including specific secretion pathways, autolysis, and membrane vesicle formation.