Immunohistochemical Expression of VEGF and Podoplanin in Uterine Cervical Squamous Intraepithelial Lesions

• Published in: Disease markers Vol. 2016; no. 2016; pp. 1 - 8
• Main Authors: De Góis Speck, Neila Maria, Nogueira Carvalho, Carmen Regina, Megale De Lima, Tatiana, Ribalta, Julisa Chamorro Lascasas, Focchi, Gustavo Rubino de Azevedo, Belfort-Mattos, Patrícia Napoli, Kesselring Tso, Fernanda
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2016; John Wiley & Sons, Inc
• Subjects: Adult; Biomarkers, Tumor - metabolism; Care and treatment; Case-Control Studies; Cervical cancer; Cervical Intraepithelial Neoplasia - metabolism; Cervical Intraepithelial Neoplasia - pathology; Cross-Sectional Studies; Development and progression; Female; Follow-Up Studies; Gene expression; Genetic aspects; Health aspects; Humans; Immunoenzyme Techniques; Membrane Glycoproteins - metabolism; Neoplasm Staging; Prognosis; Retrospective Studies; Squamous Intraepithelial Lesions of the Cervix - metabolism; Squamous Intraepithelial Lesions of the Cervix - pathology; Uterine Cervical Neoplasms - metabolism; Uterine Cervical Neoplasms - pathology; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - metabolism
• ISSN: 0278-0240; 1875-8630
• DOI: 10.1155/2016/8293196

VEGF and podoplanin (PDPN) have been identified as angiogenesis and/or lymphangiogenesis regulators and might be essential to restrict tumor growth, progression, and metastasis. In the present study, we evaluate the association between the expression of these markers and CIN grade. Immunohistochemistry was performed in 234 uterine cervical samples using conventional histologic sections or TMA with the monoclonal antibodies to VEGF (C-1 clone) and podoplanin (D2-40 clone). Positive-staining rates of VEGF in 191 CIN specimens were significantly associated with histological grade (P<0.001). Negative and/or focal immunostaining for PDPN were more frequent in CIN 3 (P=0.016). We found that patients with CIN 3 more frequently had strong and more diffuse staining for VEGF and diminished staining for PDPN (P=0.018). Strong and more diffuse VEGF immunoexpressions in CIN 2 and CIN 3 were detected when compared to CIN 1. Negative and/or focal PDPN immunoexpression appear to be more frequent in CIN 3. Moderate to strong VEGF expression may be a tendency among patients with high-grade lesions and diminished PDPN expression.