PCR Amplification and Phylogenetic Analysis of groESL Operon Sequences from Ehrlichia ewingii and Ehrlichia muris

• Published in: Journal of clinical microbiology Vol. 38; no. 7; pp. 2746 - 2749
• Main Authors: Sumner, John W., Storch, Gregory A., Buller, Richard S., Liddell, Allison M., Stockham, Steven L., Rikihisa, Yasuko, Messenger, Sharon, Paddock, Christopher D.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.07.2000
• Series: Note
• Subjects: Animals; Bacterial Proteins - genetics; Bacteriology; Biological and medical sciences; Cells, Cultured; Chaperonins - genetics; Chlamydiology and Rickettsiology; DNA, Bacterial - genetics; Dogs; Ehrlichia - classification; Ehrlichia - genetics; Ehrlichia ewingii; Ehrlichia muris; Ehrlichiosis - microbiology; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Genetics; groESL operon; Humans; Mice; Microbiology; Molecular Sequence Data; Operon; Phylogeny; Polymerase Chain Reaction - methods; RNA, Ribosomal, 16S - genetics; rRNA 16S gene; Sequence Analysis, DNA; Ehrlichiaceae; Rickettsiales; Nucleotide sequence; Operon; Bacteria; Identification; Species specificity; Phylogeny
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/JCM.38.7.2746-2749.2000

Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii , recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris . Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.