PCR Amplification and Phylogenetic Analysis of groESL Operon Sequences from Ehrlichia ewingii and Ehrlichia muris

Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii , recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris . Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comp...

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Published inJournal of clinical microbiology Vol. 38; no. 7; pp. 2746 - 2749
Main Authors Sumner, John W., Storch, Gregory A., Buller, Richard S., Liddell, Allison M., Stockham, Steven L., Rikihisa, Yasuko, Messenger, Sharon, Paddock, Christopher D.
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.07.2000
SeriesNote
Subjects
Animals
Bacterial Proteins - genetics
Bacteriology
Biological and medical sciences
Cells, Cultured
Chaperonins - genetics
Chlamydiology and Rickettsiology
DNA, Bacterial - genetics
Dogs
Ehrlichia - classification
Ehrlichia - genetics
Ehrlichia ewingii
Ehrlichia muris
Ehrlichiosis - microbiology
Fundamental and applied biological sciences. Psychology
Genes, rRNA
Genetics
groESL operon
Humans
Mice
Microbiology
Molecular Sequence Data
Operon
Phylogeny
Polymerase Chain Reaction - methods
RNA, Ribosomal, 16S - genetics
rRNA 16S gene
Sequence Analysis, DNA
Ehrlichiaceae
Rickettsiales
Nucleotide sequence
Operon
Bacteria
Identification
Species specificity
Phylogeny
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Summary:Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii , recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris . Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.
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Corresponding author. Mailing address: Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd., MS/G13, Atlanta, GA 30033. Phone: (404) 639-1097. Fax: (404) 639-4436. E-mail: jws3@cdc.gov.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.38.7.2746-2749.2000