Glycosaminoglycans detection methods: Applications of mass spectrometry

Glycosaminoglycans (GAGs) are long blocks of negatively charged polysaccharides. They are one of the major components of the extracellular matrix and play multiple roles in different tissues and organs. The accumulation of undegraded GAGs causes mucopolysaccharidoses (MPS). GAGs are associated with...

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Published inMolecular genetics and metabolism Vol. 120; no. 1-2; pp. 67 - 77
Main Authors Kubaski, Francyne, Osago, Harumi, Mason, Robert W, Yamaguchi, Seiji, Kobayashi, Hironori, Tsuchiya, Mikako, Orii, Tadao, Tomatsu, Shunji
Format Journal Article
LanguageEnglish
Published United States 01.01.2017
Subjects
Chromatography, Thin Layer
Early Diagnosis
Glycosaminoglycans - metabolism
Humans
Mucopolysaccharidoses - diagnosis
Mucopolysaccharidoses - metabolism
Tandem Mass Spectrometry
Translational Medical Research
Glycosaminoglycans
Mass spectrometry
Alcian blue
Chromatography, mucopolysaccharidoses
ELISA
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Summary:Glycosaminoglycans (GAGs) are long blocks of negatively charged polysaccharides. They are one of the major components of the extracellular matrix and play multiple roles in different tissues and organs. The accumulation of undegraded GAGs causes mucopolysaccharidoses (MPS). GAGs are associated with other pathological conditions such as osteoarthritis, inflammation, diabetes mellitus, spinal cord injury, and cancer. The need for further understanding of GAG functions and mechanisms of action boosted the development of qualitative and quantitative (alcian blue, toluidine blue, paper and thin layer chromatography, gas chromatography, high pressure liquid chromatography, capillary electrophoresis, 1,9-dimethylmethylene blue, enzyme linked-immunosorbent assay, mass spectrometry) techniques. The availability of quantitative techniques has facilitated translational research on GAGs into the medical field for: 1) diagnosis, monitoring, and screening for MPS; 2) analysis of GAG synthetic and degradation pathways; and 3) determination of physiological and pathological roles of GAGs. This review provides a history of development of GAG assays and insights about the use of tandem mass spectrometry and its applications for GAG analysis.
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The first three authors should be regarded as joint first authors.
ISSN:1096-7192
1096-7206
DOI:10.1016/j.ymgme.2016.09.005