Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)

• Published in: Pure and applied chemistry Vol. 85; no. 3; pp. 589 - 608
• Main Authors: Ameloot, Marcel, vandeVen, Martin, Acuña, A. Ulises, Valeur, Bernard
• Format: Journal Article
• Language: English
• Published: Berlin De Gruyter 11.01.2013; Walter de Gruyter GmbH
• Subjects: Anisotropy; Fluorescence; Fluorimetry; IUPAC Analytical Chemistry Division; IUPAC Organic and Biomolecular Chemistry Division; IUPAC Physical and Biophysical Chemistry Division; Measurement methods; photoluminescence; Polarization; Reference materials; standardization

After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample...