Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)

After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample...

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Published inPure and applied chemistry Vol. 85; no. 3; pp. 589 - 608
Main Authors Ameloot, Marcel, vandeVen, Martin, Acuña, A. Ulises, Valeur, Bernard
Format Journal Article
LanguageEnglish
Published Berlin De Gruyter 11.01.2013
Walter de Gruyter GmbH
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Summary:After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample properties. A list of selected reference values of steady-state fluorescence anisotropy and polarization is given. Attention is also paid to analysis of time-resolved fluorescence anisotropy data obtained by pulse fluorometry or phase and modulation fluorometry techniques. Recommendations for checking the accuracy of measurements are provided together with a list of selected time-resolved fluorescence anisotropy data as reported in the literature.
ISSN:0033-4545
1365-3075
DOI:10.1351/PAC-REP-11-11-12