Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)

After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample...

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Bibliographic Details
Published inPure and applied chemistry Vol. 85; no. 3; pp. 589 - 608
Main Authors Ameloot, Marcel, vandeVen, Martin, Acuña, A. Ulises, Valeur, Bernard
Format Journal Article
LanguageEnglish
Published Berlin De Gruyter 11.01.2013
Walter de Gruyter GmbH
Subjects
Anisotropy
Fluorescence
Fluorimetry
IUPAC Analytical Chemistry Division
IUPAC Organic and Biomolecular Chemistry Division
IUPAC Physical and Biophysical Chemistry Division
Measurement methods
photoluminescence
Polarization
Reference materials
standardization
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Summary:After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample properties. A list of selected reference values of steady-state fluorescence anisotropy and polarization is given. Attention is also paid to analysis of time-resolved fluorescence anisotropy data obtained by pulse fluorometry or phase and modulation fluorometry techniques. Recommendations for checking the accuracy of measurements are provided together with a list of selected time-resolved fluorescence anisotropy data as reported in the literature.
ISSN:0033-4545
1365-3075
DOI:10.1351/PAC-REP-11-11-12