Sodium Mercaptoethane Sulfonate Reduces Collagenolytic Degradation and Synergistically Enhances Antimicrobial Durability in an Antibiotic-Loaded Biopolymer Film for Prevention of Surgical-Site Infections

Implant-associated surgical-site infections can have significant clinical consequences. Previously we reported a method for prophylactically disinfecting implant surfaces in surgical pockets, where an antibiotic solution containing minocycline (M) and rifampin (R) was applied as a solid film in a cr...

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Published inBioMed research international Vol. 2017; no. 2017; pp. 1 - 8
Main Authors Selber, Jesse, Vargas-Cruz, Nylev, Viola, George M., Reitzel, Ruth, Rosenblatt, Joel, Raad, Issam
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 01.01.2017
Hindawi
Hindawi Limited
Subjects
Antibiotics
Antiinfectives and antibacterials
Antimicrobial agents
Biochemistry
Biofilms
Biomedical research
Biopolymers
Cancer
Collagen
Colonization
Crosslinking
Durability
Enzymes
Fibroblasts
Glycerol
Incubation
Infections
Inflammation
Medical equipment
Methicillin
Minocycline
Multidrug resistance
Prophylaxis
Proteolysis
Pseudomonas aeruginosa
Rifampin
Silicones
Sodium
Studies
Surgery
Surgical implants
Surgical site infections
Transplants & implants
Viability
United States > US
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Summary:Implant-associated surgical-site infections can have significant clinical consequences. Previously we reported a method for prophylactically disinfecting implant surfaces in surgical pockets, where an antibiotic solution containing minocycline (M) and rifampin (R) was applied as a solid film in a crosslinked biopolymer matrix that partially liquefied in situ to provide extended prophylaxis. Here we studied the effect of adding sodium 2-mercaptoethane sulfonate (MeSNA) on durability of prophylaxis in an in vitro model of implant-associated surgical-site infection. Adding MeSNA to the M/R biopolymer, antimicrobial film extended the duration for which biofilm formation by multidrug-resistant Pseudomonas aeruginosa (MDR-PA) was prevented on silicone surfaces in the model. M/R films with and without MeSNA were effective in preventing colonization by methicillin-resistant Staphylococcus aureus. Independent experiments revealed that MeSNA directly inhibited proteolytic digestion of the biopolymer film and synergistically enhanced antimicrobial potency of M/R against MDR-PA. Incubation of the MeSNA containing films with L929 fibroblasts revealed no impairment of cellular metabolic activity or viability.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Academic Editor: Hyuk Sang Yoo
ISSN:2314-6133
2314-6141
DOI:10.1155/2017/3149536