Comprehensive Gene Expression Analysis of Cerebral Cortices from Mature Rats After Neonatal Hypoxic–Ischemic Brain Injury

• Published in: Journal of molecular neuroscience Vol. 49; no. 2; pp. 320 - 327
• Main Authors: Kojima, Toshio, Ueda, Yuto, Sato, Akira, Sameshima, Hiroshi, Ikenoue, Tsuyomu
• Format: Journal Article
• Language: English
• Published: New York Humana Press Inc 01.02.2013; Springer Nature B.V
• Subjects: Animals; Arachidonic Acid - metabolism; Biomedical and Life Sciences; Biomedicine; Brain research; Cell Biology; Cell cycle; Cell Death - genetics; Cerebral Cortex - metabolism; Gene expression; Gene Expression Profiling; Gene Regulatory Networks; Hypoxia; Hypoxia-Ischemia, Brain - metabolism; Hypoxia-Ischemia, Brain - pathology; Medical research; Medicine; Neurochemistry; Neurology; Neurosciences; Oligonucleotide Array Sequence Analysis; Oryza sativa; Proteomics; Rats; Rats, Wistar; RNA, Messenger - biosynthesis; RNA, Messenger - metabolism; Signal Transduction - genetics; Systems development; Traumatic brain injury; Up-Regulation; Japan; Neonatal hypoxic–ischemic encephalopathy; DNA microarray; Gene expression; Gene network
• ISSN: 0895-8696; 1559-1166
• DOI: 10.1007/s12031-012-9830-5

Neonatal hypoxic–ischemic (HI) encephalopathy can lead to severe brain damage and is a common cause of neurological handicaps in adulthood. To elucidate the molecular events occurring in cerebral cortices of mature rats (8 weeks old) after neonatal HI brain insult, we performed comprehensive gene expression and gene network analyses using a DNA microarray system (Agilent 4x44K). A rat model of neonatal HI encephalopathy (Rice model) was obtained by unilateral ligation of the common carotid artery of 7-day-old rats with hypoxia (exposure to 8 % oxygen). Due to the HI insult-related breakdown of the ipsilateral hemisphere in the brain, RNAs were prepared from the contralateral cerebral cortices of 8-week-old rats and analyzed by DNA microarray. Biofunctional analysis of differentially regulated genes revealed that many upregulated genes were related to cell death signaling, such as the arachidonic acid cascade. In contrast, many downregulated genes were related to gene expression, reflecting progressive damage by the HI insult, even within the contralateral cerebral hemisphere.