Zoledronic acid inhibits proliferation and impairs migration and invasion through downregulating VEGF and MMPs expression in human nasopharyngeal carcinoma cells

• Published in: Medical oncology (Northwood, London, England) Vol. 29; no. 2; pp. 714 - 720
• Main Authors: Li, Xu-yuan, Lin, Ying-cheng, Huang, Wan-lan, Hong, Chao-qun, Chen, Jiong-yu, You, Yan-jie, Li, Wei-bing
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.06.2012; Springer Nature B.V
• Subjects: Blotting, Western; Bone Density Conservation Agents - pharmacology; Carcinoma; Cell Adhesion - drug effects; Cell Movement - drug effects; Cell Proliferation - drug effects; Diphosphonates - pharmacology; Down-Regulation; Enzyme-Linked Immunosorbent Assay; Hematology; Humans; Imidazoles - pharmacology; Internal Medicine; Matrix Metalloproteinase 2 - genetics; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 9 - genetics; Matrix Metalloproteinase 9 - metabolism; Medicine; Medicine & Public Health; Nasopharyngeal Carcinoma; Nasopharyngeal Neoplasms - genetics; Nasopharyngeal Neoplasms - metabolism; Nasopharyngeal Neoplasms - pathology; Neoplasm Invasiveness; Oncology; Original Paper; Pathology; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Tumor Cells, Cultured; Vascular Endothelial Growth Factor A - genetics; Vascular Endothelial Growth Factor A - metabolism; Zoledronic Acid; Zoledronic acid; Matrix metalloproteinases; Vascular endothelial growth factor; Migration; Invasion; Human nasopharyngeal carcinoma cell line
• ISSN: 1357-0560; 1559-131X
• DOI: 10.1007/s12032-011-9904-1

To demonstrate the effect of zoledronic acid in proliferation, invasion, and migration of human nasopharyngeal carcinoma cell HNE-1 and explore the potential role of VEGF, MMP-2, and MMP-9 proteins in vitro. Human nasopharyngeal carcinoma cell HNE-1 was exposed to various concentrations (0–40 μmol/l) of zoledronic acid. Zoledronic acid inhibited proliferation of HNE-1 cells though not in a dose-dependent manner. Zoledronic acid had exerted a dose-dependent effect on the migration and invasion of HNE-1 cells. Both expressions of mRNA and protein of MMP2, MMP9, and VEGF were reduced, respectively, detected by RT-PCR and Western blot assays. These data suggested that zoledronic acid not only inhibited growth but also invasion and migration of HNE-1 cells in vitro. The anti-cancer action of zoledronic acid was partially associated with the suppression of VEGF expression and secretion and downregulating the expression of MMP2 and MMP9.