Optimization of Ultrasound-Assisted Extraction of phenolic compounds and anthocyanins from blueberry (Vaccinium ashei) wine pomace

• Published in: Food chemistry Vol. 204; pp. 70 - 76
• Main Authors: He, Bo, Zhang, Ling-Li, Yue, Xue-Yang, Liang, Jin, Jiang, Jun, Gao, Xue-Ling, Yue, Peng-Xiang
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2016
• Subjects: anthocyanins; Anthocyanins - isolation & purification; blueberries; Blueberry Plants - chemistry; Blueberry Wine Pomace; Conventional Solvent Extraction; Food Handling; Glucosides - isolation & purification; Model-based optimization; phenolic compounds; Phenols - isolation & purification; Plant Extracts - analysis; pomace; Response Surface Methodology; solvents; Tandem Mass Spectrometry; temperature; Total anthocyanins; Total phenolics; ultrasonic treatment; Ultrasonics - methods; Ultrasound-Assisted Extraction; Vaccinium; Vaccinium ashei; Vaccinium virgatum; Vitaceae; Wine - analysis; wines; Total anthocyanins; Response Surface Methodology; Blueberry Wine Pomace; Total phenolics; Conventional Solvent Extraction; Ultrasound-Assisted Extraction; Model-based optimization
• ISSN: 0308-8146; 1873-7072; 1873-7072
• DOI: 10.1016/j.foodchem.2016.02.094

•Bioactive compounds were extracted from byproduct Blueberry Wine Pomace.•Ultrasound-Assisted Extraction technique was optimized for phenolics and anthocyanins recovery.•Ultrasound-Assisted Extraction yielded more than Conventional Solvent Extraction.•HPLC–DAD–MS/MS showed that the two methods yielded the same anthocyanins. Ultrasound-Assisted Extraction (UAE) of total anthocyanins (TA) and phenolics (TP) from Blueberry Wine Pomace (BWP) was optimized using Response Surface Methodology (RSM). A Box–Behnken design was used to predict that the optimized conditions were an extraction temperature of 61.03°C, a liquid–solid ratio of 21.70mL/g and a sonication time of 23.67min. Using the modeled optimized conditions, the predicted and experimental yields of TA and TP were within a 2% difference. The yields of TA and TP obtained through the optimized UAE method were higher than those using a Conventional Solvent Extraction (CSE) method. Seven anthocyanins, namely delphinidin-3-O-glucoside, delphindin-3-O-arabinoside, petunidin-3-O-glucoside, cyanidin-3-O-arabinoside, cyanidin-3-O-glucoside, malvidin-3-O-glucoside and malvidin-3-O-arabinoside, were found in the BWP extract from both the UAE and CSE methods.