Mapping and introgression of a tomato yellow leaf curl virus tolerance gene, TY-1

• Published in: Theoretical and applied genetics Vol. 88; no. 2; p. 141
• Main Authors: Zamir, D, Ekstein-Michelson, I, Zakay, Y, Navot, N, Zeidan, M, Sarfatti, M, Eshed, Y, Harel, E, Pleban, T, Oss, H. van, Kedar, N, Rabinowitch, H.D, Czosnek, H. (Jerusalem Univ., Rehovot (Israel). Faculty of Agriculture. Dept. of Field and Vegetable Crops)
• Format: Journal Article
• Language: English
• Published: Germany 01.05.1994
• Subjects: CARTE GENETIQUE; GEMINIVIRUS ENROULEMENT JAUNE TOMAT; GEMINIVIRUS RIZADO AMARILLO TOMATE; GENE; GENES; LYCOPERSICON; MAPAS GENETICOS; RESISTANCE AUX MALADIES; RESISTENCIA A LA ENFERMEDAD; SELECCION; SELECTION
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/bf00225889

The whitefly-transmitted tomato yellow-leaf curl gemini-virus (TYLCV) is a major pathogen of tomatoes. The wild tomato species Lycopersicon chilense, which is resistant to the virus, was crossed to the cultivated tomato, L. esculentum. The backcross-1 selfed (BC1S1) generation was inoculated and a symptomless plant was selected. This plant was analyzed using 61 molecular markers, which span the tomato genome, to determine which L. chilense chromosome segments were introgressed. A BC2S1 population was cage-inoculated with viroliferous whiteflies (Bemisia tabaci), the natural insect vector of the virus, and subjected to RFLP analysis. Markers on chromosomes 3 and 6 were significantly associated with the level of tolerance; the association of chromosome-6 markers was further substantiated in two additional BC2S1 populations. A tolerant BC2S1 plant which was homozygous for L. chilense introgressions in chromosomes 3, 6 and 7 was crossed to generate a BC3S1 population which was planted in an infested field. A TYLCV-tolerance gene with partial dominance, TY-1, was mapped to chromosome 6; two modifier genes were mapped to chromosomes 3 and 7. Field and whitefly-mediated cage inoculations of nearly-isogenic lines in BC3S3 supported our conclusion that TY-1 is the major TYLCV-tolerance locus.