Paraventricular nucleus bicuculline alters frequency components of sympathetic nerve discharge bursts
Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas 66506; and Department of Physiology and Biophysics, University of Nebraska Medical Center, Omaha, Nebraska 68198 Autospectral and coherence analyses were used to determine the effect of paraventricular nucleus (PVN) GAB...
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Published in | American journal of physiology. Heart and circulatory physiology Vol. 281; no. 3; pp. H1233 - H1241 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.09.2001
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Subjects | |
Online Access | Get full text |
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Summary: | Department of Anatomy and Physiology, Kansas State University,
Manhattan, Kansas 66506; and Department of Physiology and
Biophysics, University of Nebraska Medical Center, Omaha, Nebraska
68198
Autospectral and coherence
analyses were used to determine the effect of paraventricular nucleus
(PVN) GABA A receptor antagonism [microinfusion or
microinjections of bicuculline methiodide (BMI) 100 pmoles] on
sympathetic nerve discharge (SND) frequency components (bursting
pattern and relationships between discharges in regionally selective
nerves) in -chloralose-anesthetized rats. SND was recorded from the renal, splenic, and lumbar nerves. The following observations were made. First, PVN BMI microinjections, but not PVN saline or
cortical BMI microinjections, transformed the cardiac-related SND
bursting pattern in baroreceptor-innervated rats to one characterized by the presence of low-frequency bursts not synchronized to the cardiac
cycle or phrenic nerve discharge bursts. Second, SND pattern changes
were similar in the renal, splenic, and lumbar nerves, and peak
coherence values relating low-frequency bursts in sympathetic nerve
pairs (renal-splenic, renal-lumbar, and splenic-lumbar) were
significantly increased from preinjection control after PVN BMI
microinjection. Third, PVN BMI microinjections significantly increased
the coupling between low-frequency SND bursts in
baroreceptor-denervated rats. Finally, PVN BMI-induced changes in the
SND bursting pattern were not observed after PVN pretreatment with
muscimol (GABA agonist, 1 nmole). We conclude that PVN
GABA A receptor antagonism profoundly alters the frequency
components in sympathetic nerves.
sympathetic nerve activity; autospectral analysis; coherence
function; Sprague-Dawley rats |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0363-6135 1522-1539 |
DOI: | 10.1152/ajpheart.2001.281.3.h1233 |