Continuous Renal Replacement Therapy (CRRT) Attenuates Myocardial Inflammation and Mitochondrial Injury Induced by Venovenous Extracorporeal Membrane Oxygenation (VV ECMO) in a Healthy Piglet Model

• Published in: Inflammation Vol. 36; no. 5; pp. 1186 - 1193
• Main Authors: Shen, Juanhong, Yu, Wenkui, Chen, Qiyi, Shi, Jialiang, Hu, Yimin, Zhang, Juanjuan, Gao, Tao, Xi, Fengchan, He, Changsheng, Gong, Jianfeng, Li, Ning, Li, Jieshou
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.10.2013; Springer Nature B.V
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Cardiomyopathies - immunology; Cardiomyopathies - pathology; Electron Transport Complex I - metabolism; Electron Transport Complex IV - metabolism; Extracorporeal Membrane Oxygenation - adverse effects; Gene expression; Immunology; Inflammation - immunology; Inflammation - metabolism; Interleukin-1beta - blood; Interleukin-1beta - genetics; Interleukin-1beta - metabolism; Interleukin-6 - blood; Interleukin-6 - genetics; Interleukin-6 - metabolism; Internal Medicine; Mitochondria - pathology; Myocardium - pathology; Pathology; Peroxidase - metabolism; Pharmacology/Toxicology; Renal Replacement Therapy - adverse effects; Rheumatology; RNA, Messenger - biosynthesis; Swine; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism; myocardial inflammation; continuous renal replacement therapy (CRRT); mitochondrial function; venovenous extracorporeal membrane oxygenation (VV ECMO)
• ISSN: 0360-3997; 1573-2576
• DOI: 10.1007/s10753-013-9654-7

In this study, we investigated the myocardial inflammation and mitochondrial function during venovenous extracorporeal membrane oxygenation (VV ECMO) and further evaluated the effects of continuous renal replacement therapy (CRRT) on them. Eighteen piglets were assigned to the control group, ECMO group, and ECMO+CRRT group. Myocardial inflammation was assessed by the activity of myeloperoxidase (MPO), myocardial concentrations, and mRNA expression of TNF-α, IL-1β, and IL-6; mitochondrial function was assessed by activities of mitochondrial complexes I–V. VV ECMO elicited a general activation of serum and myocardial inflammation and significantly decreased the activities of mitochondrial complexes I and IV. After being combined with CRRT, serum and myocardial concentrations of IL-1β and IL-6, myocardial mRNA expression of IL-6, and the activity of MPO were decreased significantly; the activities of mitochondrial complexes were increased. We conclude that myocardial inflammation was activated during ECMO therapy, inducing mitochondrial injury; moreover, CRRT reduced myocardial inflammation and partially ameliorated mitochondrial function.