Regional expression of the BCRP/ABCG2 transporter in term human placentas

• Published in: Reproductive toxicology (Elmsford, N.Y.) Vol. 43; pp. 72 - 77
• Main Authors: Memon, Naureen, Bircsak, Kristin M., Archer, Faith, Gibson, Christopher J., Ohman-Strickland, Pamela, Weinberger, Barry I., Parast, Mana M., Vetrano, Anna M., Aleksunes, Lauren M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2014
• Subjects: ABCG2; Adult; ATP Binding Cassette Transporter, Sub-Family G, Member 2; ATP-Binding Cassette Transporters - metabolism; BCRP; Female; Housekeeping gene; Humans; Neoplasm Proteins - metabolism; Placenta; Placenta - metabolism; Placenta - pathology; Pregnancy; RNA, Messenger - metabolism; Transporter; BCRP; PBS; ABC; RPL13a; PRL; 18S; Ct; Placenta; cDNA; Housekeeping gene; GAPDH; Transporter; ABCG2
• ISSN: 0890-6238; 1873-1708
• DOI: 10.1016/j.reprotox.2013.11.003

•BCRP mRNA and protein are uniform across the term human placental disc.•GAPDH mRNA was the most appropriate housekeeping gene for expression of BCRP mRNA.•Single site sampling can be used to assess the interindividual regulation of BCRP. The breast cancer resistance protein (BCRP, ABCG2) is an efflux transporter that removes xenobiotics that cross the placenta back to the maternal circulation, thereby limiting exposure of the fetus to drugs and chemicals. Currently, variability of BCRP expression within the placenta is not known. Ten placentas were collected from healthy women undergoing elective Cesarean sections at term. Villous samples were dissected in defined regions (medial, intermediate, and peripheral) and BCRP mRNA and protein were quantified. There were no regional differences in mRNA expression of housekeeping genes (GAPDH, RPL13a, PRL, 18S). GAPDH had the lowest correlation with BCRP Ct values and was used for BCRP mRNA normalization. No differences in placental BCRP mRNA and protein were observed among the sample sites (<20% variability). Sampling site does not affect the expression of BCRP, supporting the utility of single site sampling protocols to assess the interindividual regulation of this transporter in human placentas.