Performance of recombinant proteins in diagnosis and differentiation of canine visceral leishmaniasis infected and vaccinated dogs

Control of canine visceral leishmaniasis (CVL), a major zoonotic disease in Brazil and many other tropical and subtropical countries, remains difficult as an accurate and reliable diagnosis is still missing. In endemic regions, infected dogs are the main parasitic reservoir host of human Visceral le...

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Published inEuropean journal of microbiology & immunology Vol. 10; no. 3; pp. 165 - 171
Main Authors Pereira, Ingrid E, Silva, Kyssia P, Menegati, Laura M, Pinheiro, Aimara C, Assuncao, Elaine A.O, Araujo, Maria De Lourdes P, Abass, Elfadil, Duthie, MaLcolm S, Steinhoff, Ulrich, Teixeira, Henrique C
Format Journal Article
LanguageEnglish
Published Budapest Akademiai Kiado 01.09.2020
Akadémiai Kiadó
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Summary:Control of canine visceral leishmaniasis (CVL), a major zoonotic disease in Brazil and many other tropical and subtropical countries, remains difficult as an accurate and reliable diagnosis is still missing. In endemic regions, infected dogs are the main parasitic reservoir host of human Visceral leishmaniasis (VL) infection. Vaccination of dogs against Leishmania infection constitutes an important strategy to prevent or to better control CVL, thus, a serological test that can discriminate between antibodies induced by immunization versus infection is highly desirable in order to improve and simplify diagnosis. Here, four recombinant proteins were evaluated for their ability to detect and differentiate between dogs that are infected with Leishmania or have been immunized with the anti-Leishmania vaccine Leish-Tec[R]. Receiver operating characteristic (ROC) curve analysis of the four Leishmania-specific IgG ELISA revealed superior performance of rK28, followed by rKL08, rK39 and rLb6H. The rK28-based ELISA revealed not only the best accuracy against CVL, but also the lowest cross-reactivity with sera from Leish-Tec[R] immunized dogs. Our data show that the rK28-based ELISA is highly suitable for CVL screening as it shows high sensitivity with simultaneous low cross-reactivity. Further, the high specificity of the rKL08 indicates its suitability for the confirmation of CVL diagnosis.
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HCT and US contributed equally to this work and share last authorship.
ISSN:2062-509X
2062-8633
DOI:10.1556/1886.2020.00018