Infrared Spectroscopy as a Tool to Study the Antioxidant Activity of Polyphenolic Compounds in Isolated Rat Enterocytes

• Published in: Oxidative medicine and cellular longevity Vol. 2016; no. 2016; pp. 1 - 10
• Main Authors: Alvarez-Parrilla, Emilio, Pérez-León, Jorge A., Martínez Martínez, Alejandro, de la Rosa, Laura A., Castillo-Michel, Hiram, Barraza-Garza, Guillermo, Cotte, Marine
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2016; Hindawi Limited
• Subjects: Animals; Antibiotics; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Cell Survival - drug effects; Enterocytes - chemistry; Enterocytes - cytology; Enterocytes - drug effects; Hydrochloric acid; Hydrogen Peroxide - toxicity; Intestines - cytology; Laboratory animals; Lipid Peroxidation - drug effects; Lipids; Oxidation; Oxidative stress; Oxidative Stress - drug effects; Polyphenols; Polyphenols - chemistry; Polyphenols - pharmacology; Principal Component Analysis; Principal components analysis; Rats; Rats, Sprague-Dawley; Rodents; Spectroscopy, Fourier Transform Infrared; Spectrum analysis
• ISSN: 1942-0900; 1942-0994
• DOI: 10.1155/2016/9245150

The protective effect of different polyphenols, catechin (Cat), quercetin (Qc) (flavonoids), gallic acid (GA), caffeic acid (CfA), chlorogenic acid (ChA) (phenolic acids), and capsaicin (Cap), against H2O2-induced oxidative stress was evaluated in rat enterocytes using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) Spectroscopy and Fourier Transform Infrared Microspectroscopy (FTIRM), and results were compared to standard lipid peroxidation techniques: conjugated dienes (CD) and Thiobarbituric Acid Reactive Substances (TBARS). Analysis of ATR-FTIR and FTIRM spectral data allowed the simultaneous evaluation of the effects of H2O2 and polyphenols on lipid and protein oxidation. All polyphenols showed a protective effect against H2O2-induced oxidative stress in enterocytes, when administered before or after H2O2. Cat and capsaicin showed the highest protective effect, while phenolic acids had weaker effects and Qc presented a mild prooxidative effect (IR spectral profile of biomolecules between control and H2O2-treated cells) according to FTIR analyses. These results demonstrated the viability to use infrared spectroscopy to evaluate the oxidant and antioxidant effect of molecules in cell systems assays.