The amelioration of streptozotocin diabetes-induced renal damage by Wu-Ling-San (Hoelen Five Herb Formula), a traditional Chinese prescription

• Published in: Journal of ethnopharmacology Vol. 124; no. 2; pp. 211 - 218
• Main Authors: Liu, I-Min, Tzeng, Thing-Fong, Liou, Shorong-Shii, Chang, Chia Ju
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 15.07.2009; Amsterdam; New York: Elsevier; Elsevier
• Subjects: Albuminuria - drug therapy; animal models; Animals; Biological and medical sciences; blood chemistry; blood glucose; Blood Glucose - metabolism; creatinine; Creatinine - metabolism; Diabetes Mellitus, Experimental - complications; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - metabolism; Diabetic Nephropathies - drug therapy; Diabetic Nephropathies - metabolism; Diabetic nephropathy; Drugs, Chinese Herbal - pharmacology; Drugs, Chinese Herbal - therapeutic use; excretion; Fibronectins - metabolism; General pharmacology; glycation; Glycation End Products, Advanced - metabolism; herbal medicines; histopathology; Hypoglycemic Agents - pharmacology; Hypoglycemic Agents - therapeutic use; immunohistochemistry; Kidney - drug effects; Kidney - metabolism; Kidney - pathology; kidney glomerulus; lipid peroxidation; Lysine - analogs & derivatives; Lysine - metabolism; Magnoliopsida; Male; Medical sciences; medicinal plants; medicinal properties; Mitochondria - metabolism; NF-kappa B - metabolism; Oriental traditional medicine; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phytotherapy; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; protective effect; Rats; Rats, Wistar; Receptor for Advanced Glycation End Products; Receptors, Immunologic - metabolism; renal function; serum albumin; STZ-diabetic rats; Thiobarbituric Acid Reactive Substances - metabolism; transcription factors; transforming growth factor beta; Transforming Growth Factor beta1 - metabolism; urine; Western blotting; Wu-Ling-San; Asia; China; ESRD; PAS; NF-kB; Cr; BSA; TBA; STZ; Diabetic nephropathy; BUN; ACEIs; UAER; SBP; CML; DAB; AGEs; RAGE; i.v; MEI; TGF; Ccr; STZ-diabetic rats; Wu-Ling-San; ARBs; ELISA; TCM; Endocrinopathy; Kidney disease; Antineoplastic agent; Urinary system disease; Rat; Pharmacognosy; Toxicity; Diabetes mellitus; Rodentia; Kidney; Medicinal plant; Concomitant disease; Vertebrata; Antibiotic; Mammalia; Urinary system; Folk medicine; Medical prescription; Animal; Plant origin; Streptozotocin
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2009.04.021

Wu-Ling-San (Hoelen Five Herb Formula) is a Chinese prescription used to promote water metabolism. The present study was undertaken to characterize the effects of Wu-Ling-San on diabetic nephropathy. Streptozotocin (STZ)-diabetic rats were orally administrated with Wu-Ling-San (0.5, 1.5, 2.5 g/(kg day)) once a day for 10 weeks. Changes in the renal function related parameters in plasma and urine were analyzed at the end of 10-week administration. Kidney was isolated for pathology histology, immunohistochemistry staining and Western blot analyses. Wu-Ling-San (2.5 g/(kg day)) partially decreased the high plasma glucose level in diabetic rats. Diabetic-dependent alterations in urinary albumin, 24-h urinary albumin excretion rate, creatinine clearance, and glomerular mesangial matrix expansion were ameliorated by Wu-Ling-San. The increased expression of nuclear factor-kB as well as transforming growth factor-β 1 and the progressive accumulation of fibronectin in kidney of diabetic rats were attenuated by Wu-Ling-San. Not only the elevated levels of advanced glycation end products (AGEs) and mitochondrial thiobarbituric acid-reactive substance, but also the higher levels of N ɛ-(carboxymethyl)lysine and receptor for AGEs in kidney of diabetic rats were ameliorated by Wu-Ling-San. : Wu-Ling-San posses an anti-diabetic property with plasma glucose lowering action accompanied with amelioration of glycation-mediated renal damage.