Bamboo Leaf Flavonoids Extracts Alleviate Oxidative Stress in HepG2 Cells via Naturally Modulating Reactive Oxygen Species Production and Nrf2‐Mediated Antioxidant Defense Responses

• Published in: Journal of food science Vol. 84; no. 6; pp. 1609 - 1620
• Main Authors: Yu, Yue, Li, Zhanming, Cao, Guangtian, Huang, Shudan, Yang, Hongshun
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.06.2019
• Subjects: antioxidant activity; antioxidant defense responses; antioxidant enzymes; Antioxidants; Apoptosis; Bamboo; bamboo leaf; bamboos; droplets; Flavonoids; Food industry; Food processing industry; Forkhead protein; human cell lines; Intervention; leaf extracts; Leaves; Lipids; Membrane potential; Mitochondria; mitochondrial membrane; Oleic acid; Oxidation; Oxidative stress; Performance indices; Plant extracts; quantitative polymerase chain reaction; Reactive oxygen species; reverse transcriptase polymerase chain reaction; Signal transduction; Western blotting; bamboo leaf; antioxidant defense responses; flavonoids; oxidative stress; oleic acid
• ISSN: 0022-1147; 1750-3841; 1750-3841
• DOI: 10.1111/1750-3841.14609

In this study, bamboo leaf flavonoids extracts (BFE) were employed to alleviate oxidative stress induced by oleic acid in HepG2 cells. Biochemical indexes, intracellular reactive oxygen species production, lipid droplets accumulation, antioxidant enzymes production, and mitochondrial membrane potential were determined to show the alleviation performance of BFE intervention (P < 0.05). Importantly, the results of qRT‐PCR and western blot determination indicated that BFE intervention upregulated the expression of Nrf2/HO‐1/NQO1 to initiate the antioxidant defense response for counteracting oxidative stress (P < 0.05). Moreover, mitochondrial membrane potential–mediated apoptosis and FOXO signaling pathway initiation caused by BFE intervention may together contribute to oxidative stress alleviation in HepG2 cells. In conclusion, these findings suggested that BFE intervention upregulated related antioxidant defense responses for preventing cells from oxidative damage. Practical Application In this study, bamboo leaf flavonoids extracts intervention upregulated related antioxidant defense responses for preventing cells from oxidative damage. These findings in bamboo leaf extracts antioxidants are a promising and innovative subject with practical applications to enhance the development of bamboo leaf extracts functional products in the food industry.