Destruxin from Metarhizium anisopliae induces oxidative stress effecting larval mortality of the polyphagous pest Spodoptera litura

• Published in: Journal of applied entomology (1986) Vol. 132; no. 1; pp. 68 - 78
• Main Authors: Sree, K. Sowjanya, Padmaja, V
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.02.2008; Blackwell Publishing Ltd; Blackwell
• Subjects: antioxidant enzymes; Biological and medical sciences; Biological control; Control; Fundamental and applied biological sciences. Psychology; lethal concentration; membrane damage; Metarhizium anisopliae (Metch.); mycotoxin; mycotoxins; Phytopathology. Animal pests. Plant and forest protection; Protozoa. Invertebrates; Spodoptera litura (Fab.); Entomopathogen; Oxidative stress; Insecta; Mycotoxin; Spodoptera litura (Fab.); antioxidant enzymes; membrane damage; Fungi; Pest; Metarhizium anisopliae; Polyphagous; Destruxin; Membrane; Fungi Imperfecti; Biopesticide; Damage; Enzyme; Mortality; Metarhizium anisopliae (Metch.); Antioxidant; Toxin; Lethal concentration; Spodoptera litura; Larva; Arthropoda; Lepidoptera; Noctuidae; Invertebrata; Microbial insecticide
• ISSN: 0931-2048; 1439-0418
• DOI: 10.1111/j.1439-0418.2007.01239.x

Nine-day-old Spodoptera litura (Fab.) larvae were treated with crude destruxin (dtx) extracted from a high-virulent (M-19) and a low-virulent (M-10) isolate of the entomopathogenic fungus, Metarhizium anisopliae (Metch.), at doses that caused 30%, 50% and 90% mortality in the treated groups after 48 h. Destruxins produced a dose-dependent decrease in the body weight of the larvae after 1, 24 and 48 h of treatment. There was a considerable hike in the activity of lipoxygenase and lipid peroxidation levels in the treated larvae with increased time of exposure to mycotoxin. The activities of total superoxide dismutase, total catalase, total peroxidase and specific ascorbate peroxidase in the larval body also registered alterations in the dtx-treated larvae, suggesting that exposure of larvae to crude dtx induces oxidative stress which is countered by the antioxidant enzymes to an extent governed by the concentration and time of treatment, beyond which the larvae succumb to the ecofriendly biotoxin.