Advances in screening enzyme inhibitors by capillary electrophoresis

• Published in: Electrophoresis Vol. 40; no. 16-17; pp. 2075 - 2083
• Main Authors: Wang, Wei‐Feng, Yang, Jun‐Li
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.08.2019
• Subjects: Capillary electrophoresis; Electrophoresis; Electrophoresis, Capillary; Enzyme activity; Enzyme Assays; Enzyme inhibitor; Enzyme Inhibitors - analysis; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - metabolism; Enzymes; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Immobilization; Inhibitors; Screening; Enzyme inhibitor; Immobilization; Screening; Capillary electrophoresis
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.201900013

Capillary electrophoresis (CE) has attracted lots of attention due to its simplicity, low sample consumption, low solvent volume, high resolution, and high speed. Based on these advantages, it has been widely used in enzyme inhibitor screening. There are two main operation modes on enzyme inhibitor screening: off‐line (precapillary enzyme assays) in which process CE was used as an analytical tool; online (in‐capillary enzyme assays) which combined the sample injection, mix, reaction, separation, and detection within a single run. Additionally, diverse of new materials were introduced to immobilize enzyme, which has been coupled with CE for the study of enzyme activity and its inhibitor screening. This review gives an overview of the developments and applications for the CE‐based enzyme inhibitor screening.