The bZIP transcription factor TabZIP15 improves salt stress tolerance in wheat

• Published in: Plant biotechnology journal Vol. 19; no. 2; pp. 209 - 211
• Main Authors: Bi, Chenxi, Yu, Yuehua, Dong, Chunhao, Yang, Yuxin, Zhai, Yiqian, Du, Fengping, Xia, Chuan, Ni, Zhiyong, Kong, Xiuying, Zhang, Lichao
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.02.2021; John Wiley and Sons Inc
• Subjects: Abiotic stress; Abscisic acid; Agricultural production; Basic-Leucine Zipper Transcription Factors - genetics; Brief Communication; Brief Communications; bZIP; Conflicts of interest; Crop production; Gene Expression Regulation, Plant; Genes; Genetic modification; Genetically modified organisms; Hybrid systems; Localization; Metabolism; Morphology; Ontology; Phenotypes; Plant breeding; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Proteins; Protoplasts; Regulatory sequences; Roles; Salinity tolerance; salt stress tolerance; Salt Tolerance - genetics; Salts; Seedlings; Stress; Stress, Physiological; Transcription activation; transcriptional factor; Transgenic plants; Triticum - genetics; Triticum - metabolism; Wheat; Yeast; Yeasts; salt stress tolerance; bZIP; wheat; transcriptional factor
• ISSN: 1467-7644; 1467-7652
• DOI: 10.1111/pbi.13453

[...]its production is greatly threatened by abiotic stresses such as salt (Khataar et al., 2018; Qi et al., 2019). [...]discovering the genes involved in abiotic stress tolerance and cultivating genetically modified plant varieties with enhanced stress tolerance are currently among the most important goals for plant breeders (Genc et al., 2019). TabZIP15 is a nuclear‐localized protein (Figure 1d) with transcriptional activation activity in yeast (Figure 1e). [...]we also found that TabZIP15 has an affinity for the ABA‐responsive element (ABRE) Cis‐element using the yeast one‐hybrid assay system (Figure 1f). Functional characterization of TabZIP15 in the regulation of salt stress in wheat. (a and b) Schematic of the TabZIP15 gene structure (a) and its putative coding protein (b). (c) Expression patterns of the TabZIP15 gene in roots at different time points after salt stress treatment at the seedling stage. (d) Subcellular localization of the TabZIP15 protein in wheat leaf protoplasts. (e) Test of the transcriptional activity of the full‐length and truncated fragments of TabZIP15. (f) TabZIP15 binds specifically to the ABRE Cis‐element in yeast cells. (g) Phenotypes of KN199 and TabZIP15 transgenic plants under normal and salt stress conditions. [...]a total of 3332 DEGs were found between KN199 and H381 plants at 0 h, including 1297 up‐regulated and 2035 down‐regulated DEGs in H381.