Genetic consequences of fragmentation in “arbor vitae,” eastern white cedar (Thuja occidentalis L.), toward the northern limit of its distribution range

• Published in: Ecology and evolution Vol. 2; no. 10; pp. 2506 - 2520
• Main Authors: Xu, Huaitong, Tremblay, Francine, Bergeron, Yves, Paul, Véronique, Chen, Cungen
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.10.2012; Blackwell Publishing Ltd
• Subjects: Bayesian analysis; Boreal forest; Cedar; Coniferous forests; Differentiation; distribution limit; Forests; Fragmentation; Gene flow; Genetic diversity; Genetic structure; Genotyping; Inbreeding; latitudinal gradient; microsatellite genotyping; northern edge; Original Research; Pollen; Population; Population differentiation; Population genetics; Population structure; Populations; Seeds; Thuja occidentalis; Trees; Canada; Quebec Canada; Boreal forest; genetic diversity; northern edge; distribution limit; latitudinal gradient; microsatellite genotyping
• ISSN: 2045-7758; 2045-7758
• DOI: 10.1002/ece3.371

We tested the hypothesis that marginal fragmented populations of eastern white cedar (EWC) are genetically isolated due to reduced pollen and gene flow. In accordance with the central‐marginal model, we predicted a decrease in population genetic diversity and an increase in differentiation along the latitudinal gradient from the boreal mixed‐wood to northern coniferous forest. A total of 24 eastern white cedar populations were sampled along the north‐south latitudinal gradient for microsatellite genotyping analysis. Positive Fis values and heterozygote deficiency were observed in populations from the marginal (Fis = 0.244; PHW = 0.0042) and discontinuous zones (Fis = 0.166; PHW = 0.0042). However, populations from the continuous zone were in HW equilibrium (Fis = −0.007; PHW = 0.3625). There were no significant latitudinal effects on gene diversity (Hs), allelic richness (AR), or population differentiation (Fst). Bayesian and NJT (neighbor‐joining tree) analyses demonstrated the presence of a population structure that was partly consistent with the geographic origins of the populations. The impact of population fragmentation on the genetic structure of EWC is to create a positive inbreeding coefficient, which was two to three times higher on average than that of a population from the continuous zone. This result indicated a higher occurrence of selfing within fragmented EWC populations coupled with a higher degree of gene exchange among near‐neighbor relatives, thereby leading to significant inbreeding. Increased population isolation was apparently not correlated with a detectable effect on genetic diversity. Overall, the fragmented populations of EWC appear well‐buffered against effects of inbreeding on genetic erosion. The impact of population fragmentation on the genetic structure of EWC is to create a positive inbreeding coefficient, which was two to three times higher on average than that of a population from the continuous zone. This result indicated a higher occurrence of selfing within fragmented EWC populations coupled with a higher degree of gene exchange among near‐neighbor relatives, thereby leading to significant inbreeding. Increased population isolation was apparently not correlated with a detectable effect on genetic diversity. Overall, the fragmented populations of EWC appear well‐buffered against effects of inbreeding on genetic erosion.