Genotyping of Candidatus Syngnamydia salmonis (chlamydiales; Simkaniaceae) co-cultured in Paramoeba perurans (amoebozoa; Paramoebidae)

• Published in: Archives of microbiology Vol. 200; no. 6; pp. 859 - 867
• Main Authors: Nylund, Are, Pistone, Dario, Trösse, Christiane, Blindheim, Steffen, Andersen, Linda, Plarre, Heidrun
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2018; Springer Nature B.V
• Subjects: Amino acids; Biochemistry; Biomedical and Life Sciences; Biotechnology; Candidatus Syngnamydia salmonis; Cell Biology; Chlamydiales; Cytoplasm; Ecology; Fish; Gene sequencing; Genomes; Genotyping; Gills; Life Sciences; Microbial Ecology; Microbiology; Original Paper; Paramoeba; rRNA 16S; Salmo salar; Salmon; onis; Chlamydiales; Simkaniaceae; Paramoeba perurans; Syngnamydia salmonis
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-018-1488-0

Candidatus Syngnamydia salmonis ( Chlamydiales, Simkaniaceae ) was described as an epitheliocystis-causing bacterium from the gills of Atlantic salmon ( Salmo salar ) in Norway. A bacterium showing 99.2% 16S rRNA identity to Cand . S. salmonis is able to multiply in Paramoeba perurans and based on the classification criteria this bacterium could represent the same species as Cand . S. salmonis. Sequencing the genome of the cultured bacterium has made it possible to fulfill the minimal standards for genetic characterization of species within the order Chlamydiales. The complete rRNA genes, the amino acid sequences of SucA, PepF, Adk, HemL, DnaA, FtsK and FabI, are presented in addition to the morphology of the Chlamydia-like morphs in the cytoplasm of P. perurans .