Bioinformatics analysis of endophytic bacteria related to berberine in the Chinese medicinal plant Coptis teeta Wall

• Published in: 3 Biotech Vol. 10; no. 3; p. 96
• Main Authors: Liu, Tian-hao, Zhang, Xiao-mei, Tian, Shou-zheng, Chen, Li-guo, Yuan, Jia-li
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.03.2020; Springer Nature B.V
• Subjects: Actinobacteria; Agriculture; Bacteria; Bacteroidetes; Berberine; Bioinformatics; Biomaterials; Biotechnology; Burkholderia; Cancer Research; Chemistry; Chemistry and Materials Science; Community composition; Coptis teeta; Correlation; Damage prevention; Endophytes; Herbal medicine; High performance liquid chromatography; Medicinal plants; Microorganisms; Mycobacterium; Nutrients; Original; Original Article; Proteobacteria; Rhizosphere; Roots; rRNA 16S; Salmonella; Soils; Stem Cells; Synthesis; Berberine; 16S rDNA sequencing; Endophytic bacteria; Wall; Metabonomics; Coptis teeta Wall
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-020-2084-y

Endophytic microorganisms absorb nutrients and prevent pathogen damage, supporting healthy plant growth. However, the relationship between endophytic bacteria and berberine synthesis in the medicinal plant Coptis teeta Wall. remains unclear. Herein, we explored the community composition of endophytic bacteria related to berberine in roots, stems, and leaves of wild-type and cultivated C. teeta . Endophytic bacterial communities were analyzed by 16S rRNA sequencing, and berberine content in roots was analyzed by high-performance liquid chromatography. Proteobacteria, Actinobacteria, and Bacteroidetes were the major phyla, and Mycobacterium , Salmonella , Nocardioides , Burkholderia-Paraburkholderia , and Rhizobium were the dominant genera in root, stem, and leaf tissues. Root berberine content was positively correlated with total N, total P, total K, and available K in rhizosphere soil. In addition, root berberine content was positively correlated with Microbacterium and norank_f_7B-8 , whereas soil total K was positively correlated with Microbacterium and Burkholderia-Paraburkholderia in roots. Our results demonstrated a clear correlation between dominant endophytic bacteria and berberine synthesis in C. teeta . The findings are useful for the promotion of berberine production in C. teeta via manipulation of endophytic bacteria.