Aggregatibacter actinomycetemcomitans serotypes and DGGE subtypes in Thai adults with chronic periodontitis

• Published in: Archives of oral biology Vol. 60; no. 12; pp. 1789 - 1796
• Main Authors: Pahumunto, Nuntiya, Ruangsri, Praphansri, Wongsuwanlert, Mutita, Piwat, Supatcharin, Dahlen, Gunnar, Teanpaisan, Rawee
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2015
• Subjects: Adult; Advanced Basic Science; Aggregatibacter actinomycetemcomitans; Aggregatibacter actinomycetemcomitans - genetics; Aggregatibacter actinomycetemcomitans - isolation & purification; Asian Continental Ancestry Group - genetics; Chronic periodontitis; Chronic Periodontitis - genetics; Chronic Periodontitis - microbiology; Denaturing Gradient Gel Electrophoresis; Dentistry; DGGE; Female; Humans; Leukotoxin gene; Male; Odontologi; Polymerase Chain Reaction; Serogroup; Serotype; Serotyping; Subtype; Aggregatibacter actinomycetemcomitans; Serotype; Chronic periodontitis; Leukotoxin gene; Subtype; DGGE
• ISSN: 0003-9969; 1879-1506; 1879-1506
• DOI: 10.1016/j.archoralbio.2015.09.003

Highlights • A. actinomycetemcomitans non-serotypeable strains were predominant in Thai patients with chronic periodontitis. • Two strains of A. actinomycetemcomitans had a JP2 like deletion of the leukotoxin gene and, classified as serotype c. • Two strains of A. actinomycetemcomitans had an insertion in the leukotoxin gene and, classified as serotype c. • DGGE could classify A. actinomycetemcomitans non-serotypeable strains into 16 subtypes. • Up to 4 subtypes of A. actinomycetemcomitans could be isolated from the same deep periodontal pocket.