Effect of Hypotensive Drugs on Dynamics of Nitroxide-Producing Renal Function in Rats with Nephrogenic Hypertension

• Published in: Bulletin of experimental biology and medicine Vol. 162; no. 3; pp. 357 - 361
• Main Authors: Eliseeva, E. V., Romanchenko, E. F., Dyuizen, I. V., Tyrtyshnikova, A. V., Pigolkin, Yu. I.
• Format: Journal Article
• Language: English
• Published: New York Springer US 2017; Springer; Springer Nature B.V
• Subjects: Animals; Animals, Outbred Strains; Antihypertensive Agents - pharmacology; Benzimidazoles - pharmacology; Biomedical and Life Sciences; Biomedicine; Cell Biology; Clonidine; Clonidine - pharmacology; Furosemide; Furosemide - pharmacology; Gene Expression Regulation; Hypertension; Hypertension, Renal - drug therapy; Hypertension, Renal - genetics; Hypertension, Renal - metabolism; Hypertension, Renal - pathology; Internal Medicine; Kidney Glomerulus - drug effects; Kidney Glomerulus - metabolism; Kidney Glomerulus - pathology; Kidney Tubules, Collecting - drug effects; Kidney Tubules, Collecting - metabolism; Kidney Tubules, Collecting - pathology; Laboratory Medicine; Male; Mapping; NADP; NADPH dehydrogenase; NADPH Dehydrogenase - genetics; NADPH Dehydrogenase - metabolism; Nitric oxide; Nitric Oxide - biosynthesis; Nitric Oxide Synthase - genetics; Nitric Oxide Synthase - metabolism; Nitric-oxide synthase; Nitroxide; Pathology; Rats; Renal function; Renin - genetics; Renin - metabolism; Rodents; Time Factors; Tubules; Vasodilation; Vasodilation - drug effects; kidney; NO synthase; nitric oxide; nephrogenic hypertension
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-017-3615-3

An original model of nephrogenic hypertension in rats was used for histochemical mapping of NADPH diaphorase (NO synthase) in various renal segments to examine the effect of hypotensive drugs furosemide, bendazol, and clonidine on the time course of nitroxide production in the kidneys. In various nephron segments, these drugs modulated NO synthesis in different ways. Clonidine induced a stable up-regulation of NO synthesis, which can maintain active vasodilation and gradually diminish the rennin production. Bendazol also enhanced NO synthase activity in renal glomeruli and collecting tubules, but this effect was less pronounced and short lasting. During the first week after injection of bendazol, insignificant elevation of NO synthase activity was observed in the proximal nephron segments. Furosemide exerted the least effect on NO production in kidneys.