Effect of honeycomb film on protein adsorption, cell adhesion and proliferation

• Published in: Colloids and surfaces. A, Physicochemical and engineering aspects Vol. 284; pp. 548 - 551
• Main Authors: Sunami, Hiroshi, Ito, Emiko, Tanaka, Masaru, Yamamoto, Sadaaki, Shimomura, Masatsugu
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.08.2006
• Subjects: Cell adhesion; Cell proliferation; Fibronectin; Honeycomb film; Self-organization; Tissue engineering; Cell proliferation; Tissue engineering; Honeycomb film; Fibronectin; Cell adhesion; Self-organization
• ISSN: 0927-7757; 1873-4359
• DOI: 10.1016/j.colsurfa.2005.11.041

This article describes novel methods for controlling of cell adhesion by using micro porous polymer films. Recently we found the highly ordered micro porous films were formed when poly(ɛ-caprolactone) (PCL) solution was cast on substrates at high atmospheric humidity. The micro porous film has regular honeycomb morphology with a size of 5 μm per cell (honeycomb film). Endothelial cells grew rapidly on the honeycomb film. After 24 h cell culture, the cell number on honeycomb films was lager than that on PCL flat films. In order to elucidate the effect of honeycomb films as a scaffold for cell culture, the adsorbed proteins on honeycomb films under cell culture condition were observed. After conditioning of the honeycomb film and the flat film in DMEM containing 10% foetal bovine serum (FBS) for 72 h at 37 °C in 5% CO 2 atmosphere, the adsorbed fibronectin-FITC and albumin-Texasred on the honeycomb films was observed by using confocal laser scanning microscope (CLSM). The observation revealed that fibronectin showed site-selective adsorption behavior on the honeycomb film. Albumin adsorbed on the honeycomb film non site-selectively, while fibronectin mainly adsorbed on inside of honeycomb pores. On the flat film, fibronectin was hardly observed. Since the honeycomb film accelerate the adsorption of fibronectin which is a typical protein as a cell adhesion molecule, the film could be a scaffold with excellent cell adhesion properties.