Repellency and insecticidal activity of seven Mugwort (Artemisia argyi) essential oils against the malaria vector Anopheles sinensis

• Published in: Scientific reports Vol. 12; no. 1; p. 5337
• Main Authors: Luo, De-Yue, Yan, Zhen-Tian, Che, Lin-Rong, Zhu, Junwei Jerry, Chen, Bin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29.03.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/601/1466; 631/92; 631/92/349; Animals; Anopheles; Anopheles sinensis; Aquatic insects; Artemisia - chemistry; Artemisia argyi; Burning; Caryophyllene; Caryophyllene oxide; DEET; Distillation; Essential oils; Fumigation; Humanities and Social Sciences; Insecticides - chemistry; Insecticides - pharmacology; Larvae; Larvicides; Malaria; Malaria - prevention & control; Mosquito Vectors; Mosquitoes; multidisciplinary; Oils & fats; Oils, Volatile - chemistry; Oils, Volatile - pharmacology; Phytol; Provinces; Repellency; Science; Science (multidisciplinary); Toxicity; Vector-borne diseases; China
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-09190-0

Anopheles sinensis is the main vector of malaria with a wide distribution in China and its adjacent countries. The smoke from burning dried mugwort leaves has been commonly used to repel and kill mosquito adults especially in southern Chinese provinces. In this study, the essential oils of mugwort leaves collected from seven provinces in China were extracted by steam distillation and their chemical compositions were analyzed. Among a total of 56–87 chemical constituents confirmed by GC–MS analyses, four compounds, eucalyptol, β-caryophyllene, phytol and caryophyllene oxide, were identified with appearances from all seven distilled essential oils. The effectiveness varied in larvicidal, fumigant and repellent activities against An . sinensis from these seven essential oils with different geographic origins. The essential oil from Hubei province showed the highest larvicidal activity against the 4th instar larvae of An. sinensis , with a median lethal concentration at 40.23 µg/mL. For fumigation toxicity, essential oils from 4 provinces (Gansu, Shandong, Sichuan and Henan) were observed with less than 10 min in knockdown time. The essential oil distilled from Gansu province displayed the highest repellent activity against Anopheles mosquitoes and provided similar level of protection as observed from DEET. Eucalyptol was the most toxic fumigant compound and phytol showed the strongest larvicidal activity among all tested mugwort essential oil constituents.