Neuroprotective Effects of Isosteviol Sodium in Murine Brain Capillary Cerebellar Endothelial Cells (cerebEND) After Hypoxia
Ischemic stroke is one of the leading causes of death worldwide. It damages neurons and other supporting cellular elements in the brain. However, the impairment is not only confined to the region of assault but the surrounding area as well. In addition, it also brings about damage to the blood brain...
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Published in | Frontiers in cellular neuroscience Vol. 14; p. 573950 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Lausanne
Frontiers Research Foundation
28.10.2020
Frontiers Media S.A |
Subjects | |
Online Access | Get full text |
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Summary: | Ischemic stroke is one of the leading causes of death worldwide. It damages neurons and other supporting cellular elements in the brain. However, the impairment is not only confined to the region of assault but the surrounding area as well. In addition, it also brings about damage to the blood brain barrier (BBB) which in turn leads to microvascular failure and edema. Hence, this necessitates an on-going, continuous search for intervention strategies and effective treatment.Of late, the natural sweetener stevioside proved to exhibit neuroprotective effects and therapeutic benefits against cerebral-ischemia induced injury. Its injectable formulation, isosteviolsodium (STVNa) also demonstrated favorable results. Nonetheless, its effects on the BBB have not yet been investigated to date. As such, this present study was designed to assess the effects of STVNa in our in vitro stroke model of the BBB.The integrity and permeability of the BBB are governed and maintained by tight junction proteins (TJPs) such as claudin-5 and occludin. Our data show increased claudin-5 and occludin expression in oxygen and glucose (OGD)-deprived murine brain capillary cerebellar endothelial cells (cerebEND) after STVNa treatment. Likewise, upregulation of the transmembrane protein integrin-v was also observed. Finally, cell volume was reduced with simultaneous administration of STVNa and OGD in cerebEND cells.In neuropathologies such as stroke, the failure of cell volume control is a major feature leading to loss of cells in the penumbra as well as adverse outcomes. Our initial findings therefore point to the neuroprotective effects of STVNa at the BBB in vitro, which warrant further investigation for a possible future clinical intervention. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Egor Dzyubenko, Essen University Hospital, Germany Specialty section: This article was submitted to Cellular Neuropathology, a section of the journal Frontiers in Cellular Neuroscience Reviewed by: Nicole Louise Stone, University of Nottingham, United Kingdom; Xiaodi Chen, Women & Infants Hospital of Rhode Island, United States These authors have contributed equally to this work and share first authorship |
ISSN: | 1662-5102 1662-5102 |
DOI: | 10.3389/fncel.2020.573950 |