Antiproliferative Effect of Colonic Fermented Phenolic Compounds from Jaboticaba (Myrciaria trunciflora) Fruit Peel in a 3D Cell Model of Colorectal Cancer

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 15; p. 4469
• Main Authors: Augusti, Paula Rossini, Quatrin, Andréia, Mello, Renius, Bochi, Vivian Caetano, Rodrigues, Eliseu, Prazeres, Inês D., Macedo, Ana Catarina, Oliveira-Alves, Sheila Cristina, Emanuelli, Tatiana, Bronze, Maria Rosário, Serra, Ana Teresa
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 24.07.2021; MDPI
• Subjects: Antiproliferatives; Biological activity; Cell growth; Cell viability; Cluster analysis; Colorectal cancer; Colorectal carcinoma; Communication; Feces; Fermentation; Fruits; Gene expression; HHDP-digalloylglucose isomer; HT29 cells; hydrolysable tannins; Intestinal microflora; Metabolites; Microbiota; Myrciaria trunciflora; Phenolic compounds; Phenols; Phytochemicals; principal component analysis; Principal components analysis; Spheroids; Stem cells
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26154469

Jaboticaba is a Brazilian native berry described as a rich source of phenolic compounds (PC) with health promoting effects. PC from jaboticaba peel powder (JPP) have low intestinal bio-accessibility and are catabolized by gut microbiota. However, the biological implication of PC-derived metabolites produced during JPP digestion remains unclear. This study aimed to evaluate the antiproliferative effects of colonic fermented JPP (FJPP) in a 3D model of colorectal cancer (CRC) composed by HT29 spheroids. JPP samples fermented with human feces during 0, 2, 8, 24 or 48 h were incubated (10,000 µg mL−1) with spheroids, and cell viability was assessed after 72 h. Chemometric analyses (cluster and principal component analyses) were used to identify the main compounds responsible for the bioactive effect. The antiproliferative effect of FJPP in the CRC 3D model was increased between 8 h and 24 h of incubation, and this effect was associated with HHDP-digalloylglucose isomer and dihydroxyphenyl-γ-valerolactone. At 48 h of fermentation, the antiproliferative effect of FJPP was negligible, indicating that the presence of urolithins did not improve the bioactivity of JPP. These findings provide relevant knowledge on the role of colonic microbiota fermentation to generate active phenolic metabolites from JPP with positive impact on CRC.