Neonatal necrotizing enterocolitis rat model attenuated by a remote ischemic preconditioning in the pregnant

• Published in: Acta cirurgica brasileira Vol. 32; no. 3; pp. 236 - 242
• Main Authors: Gomes, Rúdnei de Oliveira Luciano, Artigiani, Neto, Ricardo, Guimarães, Neto, José de Freitas, Nunes, Adriana Porto, Montero, Edna Frasson de Souza, Martins, José Luiz
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01.03.2017
• Subjects: Animal. Rats; Animals; Animals, Newborn; Apoptosis; Caspase 3 - analysis; Cell Hypoxia; Disease Models, Animal; Enterocolitis; Enterocolitis, Necrotizing - pathology; Enterocolitis, Necrotizing - prevention & control; Female; Ileum - blood supply; Ileum - pathology; Immunohistochemistry; Intestinal Mucosa - blood supply; Ischemic Preconditioning - methods; Ki-67 Antigen - analysis; Necrotizing. Ischemic Preconditioning. Pregnancy; Pregnancy; Rats; Reperfusion Injury - prevention & control; Reproducibility of Results; Time Factors; Treatment Outcome
• ISSN: 0102-8650; 1678-2674; 0102-8650
• DOI: 10.1590/S0102-865020170030000008

To evaluate the effect of remote ischemic preconditioning (r-IPC) administered to pregnant rats, in the ileum of newborn rats subjected to hypoxia and reoxygenation. We used three pregnant rats and their newborn rats distributed in three groups: 1) Control (C) - Newborn rats born from a pregnant rat which did not undergo any intervention; 2) Hypoxia-Reoxygenation (H/R) - Newborn rats born from a pregnant rat which did not undergo any intervention, and were subjected to hypoxia-reoxygenation; 3) Remote Ischemic Preconditioning (r-IPC) - newborn rats born from a pregnant rat which was subjected to remote ischemic preconditioning twenty-four hours before giving birth and the newborn rats were subjected to hypoxia-reoxygenation. Segments of ileum were prepared for histological analysis by HE and immunohistochemistry by the Ki67 to evaluate cell proliferation, crypt depth and villus height and evaluation of apoptosis by cleaved caspase-3. The intensity of the lesions was lower in the r-IPC than in the H/R group, showing significant difference (p<0.01). The r-IPC group showed a higher proliferative activity compared to the H/R group (p<0.01), with deeper crypts (r-IPC > H/R - p < 0.05), and higher villi, showing significant difference (r-IPC > H/R - (p <0.01). The occurrence of apoptosis in the H/R group was lower in comparison to groups C and r-IPC, with significant difference (H/R < r-IPC; p<0.05). The remote ischemic preconditioning applied to the pregnant rat protected the ileum of newborn rats subjected to hypoxia and reoxygenation, with decreased intensity of the lesions in the ileum mucosa and preservation of proliferative activity, keeping the villus height and crypt depth similar to group C.