Antimicrobial resistance in diarrheagenic Escherichia coli from ready-to-eat foods

• Published in: Journal of food science and technology Vol. 54; no. 11; pp. 3612 - 3619
• Main Authors: Lima, Cíntia Matos, Souza, Ingrid Evelyn Gomes Lima, dos Santos Alves, Taila, Leite, Clícia Capibaribe, Evangelista-Barreto, Norma Suely, de Castro Almeida, Rogeria Comastri
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.10.2017; Springer Nature B.V
• Subjects: Ampicillin; Antibiotic resistance; Antibiotics; Antiinfectives and antibacterials; Antimicrobial agents; Antimicrobial resistance; Cereals; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chloramphenicol; Desserts; Drug resistance; E coli; Escherichia coli; Food; Food Science; Genes; Juices; Meat; Most probable number; Multidrug resistance; Nutrition; Original; Original Article; Polymerase chain reaction; Restaurants; Salads; Sauces; Soups; Studies; Subgroups; Sulfamethoxazole; Trimethoprim; Vegetables; Virulence; Virulence factors; Brazil; Salvador Brazil; RTE foods; Resistance; Antibiotic; Virulence; Escherichia coli
• ISSN: 0022-1155; 0975-8402
• DOI: 10.1007/s13197-017-2820-4

Certain subgroups of Escherichia coli have congenital or acquired virulence properties that allow them to cause a wide spectrum of disease. The aim of this study was to investigate the occurrence of diarrheagenic E. coli strains in ready-to-eat (RTE) foods produced in institutional, commercial and hotel restaurants in Salvador, Brazil. The presence of virulent isolates and antimicrobial resistance were evaluated. Four hundred forty-six samples were collected and grouped into cereals and vegetables, meat-based preparations, cooked salads, raw salads, garnishes, soups and sauces, desserts and juices. E. coli were detected using the most probable number method, the presence of virulence factors in isolates was determined by polymerase chain reaction (PCR) assays, and antibiotic resistance was analyzed using the disc diffusion method. In total, 15 isolates (3.1%) of E. coli were recovered; raw salads had the highest detection rate, 1.4%, followed by cooked salads, 0.8%; meat-based preparations, 0.4%; and cereals and vegetables, 0.4%. PCR assays showed that none of the isolates had the virulence genes cnf1, cnf2, eae , sta, lt1, stx1, stx2 or cdtB . The isolates showed resistance to nine antibiotics of the 15 tested, and the highest levels of resistance were found for sulfamethoxazole/trimethoprim, tetracycline, ampicillin, and chloramphenicol (13.3% of isolates for each antibiotic). One isolate from cooked salad had plasmid-mediated multidrug resistance to tetracycline, trimethoprim/sulfamethoxazole, ampicillin and chloramphenicol. These results suggest that RTE foods, especially raw salads, can be reservoirs of E. coli and facilitate the spread of antibiotic resistance genes to the gastrointestinal microbiota of humans.