Effects of early cold stress on gene expression in Chlamydomonas reinhardtii

• Published in: Genomics (San Diego, Calif.) Vol. 112; no. 2; pp. 1128 - 1138
• Main Authors: Li, Lun, Peng, Hai, Tan, Shenglong, Zhou, Junfei, Fang, Zhiwei, Hu, Zhangfeng, Gao, Lifen, Li, Tiantian, Zhang, Weixiong, Chen, Lihong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2020
• Subjects: Chlamydomonas reinhardtii; Chlamydomonas reinhardtii - genetics; Chlamydomonas reinhardtii - metabolism; Cold stress; Cold-Shock Response; Early signaling events; Expression pattern; Gene Expression Regulation, Plant; Plant Proteins - genetics; Plant Proteins - metabolism; Promoter; Promoter Regions, Genetic; Protein Kinases - genetics; Protein Kinases - metabolism; Promoter; Cold stress; Expression pattern; Chlamydomonas reinhardtii; Early signaling events
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2019.06.027

Cold stress imposes a great impact on the growth of nearly all photosynthetic organisms, including Chlamydomonas reinhardtii (C. reinhardtii). Despite prior studies on the mechanism of stress acclimation in plants, little has been done on the early events of cold sensing in C. reinhardtii. Here, we used C. reinhardtii as a model to study early events of cold signal transduction. By analyzing transcriptomic changes of C. reinhardtii exposed to cold, we found that 3471 genes were differentially expressed after 1 h of cold exposure. These genes were associated with a wide range of biological events and processes such as protein synthesis, cell cycle and protein kinase-based phosphorylation. Besides, the promoter of one gene (named as crAP2) which belongs to AP2/EREBP family and was significantly induced by cold was cloned, and functional analysis was conducted using GUS activity analysis through Agrobacterium-mediated transient assay in tobacco leaves. •Characterization of transcriptomic changes of algae in early response to cold•Identification and profiling of protein kinases of algae under cold stress•Promoter activity analysis of a cold responsive genes of algae in tobacco leaves