Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics

Real-time reverse-transcription polymerase chain reaction (RT-PCR) is currently the most popular method for early COVID-19 diagnostics. However, loop-mediated isothermal amplification (LAMP) is superior to real-time RT-PCR in rapidity and simplicity, since it does not require expensive laboratory eq...

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Published inApplied biochemistry and microbiology Vol. 57; no. 8; pp. 845 - 850
Main Authors Makarova, J. A., Fomicheva, K. A., Osipyants, A. I., Shkurnikov, M. Yu, Pokryshchenko, A. A., Tonevitsky, E. A., Vechorko, V. I.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 2021
Springer Nature B.V
Subjects
Biochemistry
Biomedical and Life Sciences
Coronaviruses
COVID-19
COVID-19 diagnostic tests
Gene amplification
Life Sciences
Medical Microbiology
Microbiology
Polymerase chain reaction
Problems and Prospects
Real time
COVID-19
coronavirus
SARS-CoV-2
diagnostics
LAMP
loop-mediated isothermal amplification
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Summary:Real-time reverse-transcription polymerase chain reaction (RT-PCR) is currently the most popular method for early COVID-19 diagnostics. However, loop-mediated isothermal amplification (LAMP) is superior to real-time RT-PCR in rapidity and simplicity, since it does not require expensive laboratory equipment and trained personnel. LAMP-based diagnostic kits for COVID-19 testing already exist, but corresponding tests are not yet widely available. The method has great potential for mass application. Here, we discuss the technical and methodological aspects of its widespread adoption.
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ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683821080032