Association of human cyclin E with a periodic G1-S phase protein kinase

• Published in: Science (American Association for the Advancement of Science) Vol. 257; no. 5078; pp. 1958 - 1961
• Main Authors: DULIC, V, LEES, E, REED, S. I
• Format: Journal Article
• Language: English
• Published: Washington, DC American Association for the Advancement of Science 25.09.1992; American Association for the Advancement of Science (AAAS)
• Subjects: Biological and medical sciences; CDC2-CDC28 Kinases; Cell Cycle; Cell cycle, cell proliferation; Cell physiology; Cyclin-Dependent Kinase 2; Cyclin-Dependent Kinases; Cyclins; Cyclins - metabolism; Fundamental and applied biological sciences. Psychology; HeLa Cells; Humans; Immunologic Techniques; Life Sciences; Macromolecular Substances; Molecular and cellular biology; Physiological aspects; Protamine Kinase; Protamine Kinase - metabolism; Protein Binding; Protein Kinases; Protein Kinases - metabolism; Protein Serine-Threonine Kinases; Proteins; Regulation(control); Enzymatic activity; Enzyme; Correlation analysis; Protein kinase; Cell cycle; Biosynthesis; Hela cell line
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1329201

G1 cyclins control the G1 to S phase transition in the budding yeast, Saccharomyces cerevisiae. Cyclin E was discovered in the course of a screen for human complementary DNAs that rescue a deficiency of G1 cyclin function in budding yeast. The amounts of both the cyclin E protein and an associated protein kinase activity fluctuated periodically through the human cell cycle; both were maximal in late G1 and early S phases. Cyclin E-associated kinase activity was correlated with the appearance of complexes containing cyclin E and the cyclin-dependent kinase Cdk2. Thus, the cyclin E-Cdk2 complex may constitute a human G1-S phase-specific regulatory protein kinase.