Vascular endothelial growth factor association with angiopoietin 1 promotes improvement in ventricular function after ischemic cardiomyopathy induced in mini pigs

• Published in: Acta cirurgica brasileira Vol. 33; no. 4; pp. 386 - 395
• Main Authors: Oliveira, André Lacerda de Abreu, Scheffer, Jussara Peters, Markoski, Melissa, Koche, Andreia, Balbinot, Alexsandra, Antunes, Fernanda, Kalil, Renato
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01.04.2018
• Subjects: Angiopoietin-1 - therapeutic use; Animals; Cell- and Tissue-Based Therapy - methods; Disease Models, Animal; Echocardiography; Genetic Therapy; Genetic Therapy - methods; Hemodynamics - physiology; Myocardial Infarction; Myocardial Infarction - pathology; Myocardial Infarction - physiopathology; Myocardial Infarction - therapy; Necrosis; Neovascularization, Physiologic; Reproducibility of Results; Swine; Swine, Miniature; Tissue; Treatment Outcome; Vascular Endothelial Growth Factor A - therapeutic use; Ventricular Function - physiology; Wound Healing
• ISSN: 0102-8650; 1678-2674
• DOI: 10.1590/s0102-865020180040000010

To investigate the safety and clinical, hemodynamic and tissue improvement ability in mini pigs undergoing cell and gene therapy for the treatment of acute myocardial infarction. Thirty-two mini pigs Br1 lineage, 12 months old, undergoing induction of acute myocardial infarction by occlusion of the diagonal branch of the paraconal coronary. They were divided into 4 groups: one control group and 3 treatment groups (cell therapy and gene cell therapy). Echocardiography reviews were performed on three occasions and histopathological analysis was performed after 4 weeks. Analysis of variance (ANOVA), Tukey and Wilcoxon tests, were performed. Association of vascular endothelial growth factor (VEGF) with angiopoietin1 (Ang1) presented satisfactory results in the improvement of ventricular function following ischemic cardiomyopathy in mini pigs when compared to the results of the other treated groups. The therapy with VEGF and the combination of VEGF with Ang1, promoted recovered function of the myocardium, characterized by reduced akinetic area and induction of neovascularization.