A simple method to fabricate a chitosan-gold nanoparticles film and its application in glucose biosensor

• Published in: Bioelectrochemistry (Amsterdam, Netherlands) Vol. 70; no. 2; pp. 342 - 347
• Main Authors: Du, Ying, Luo, Xi-Liang, Xu, Jing-Juan, Chen, Hong-Yuan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2007
• Subjects: Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensor; Chitosan; Chitosan - chemistry; Crystallization - methods; Electrochemical deposition; Electrochemistry - instrumentation; Electrochemistry - methods; Enzymes, Immobilized - chemistry; Equipment Design; Equipment Failure Analysis; Glucose - analysis; Glucose oxidase; Glucose Oxidase - chemistry; Gold - chemistry; Gold nanoparticles; Membranes, Artificial; Microelectrodes; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Particle Size; Reproducibility of Results; Sensitivity and Specificity; Electrochemical deposition; Glucose oxidase; Chitosan; Gold nanoparticles; Biosensor
• ISSN: 1567-5394; 1878-562X
• DOI: 10.1016/j.bioelechem.2006.05.002

A novel film of chitosan-gold nanoparticles is fabricated by a direct and facile electrochemical deposition method and its application in glucose biosensor is investigated. HAuCl 4 solution is mixed with chitosan and electrochemically reduced to gold nanoparticles, which can be stabilized by chitosan and electrodeposited onto glassy carbon electrode surfaces along with the electrodeposition of chitosan. Then a model enzyme, glucose oxidase (GOD) is immobilized onto the resulting film to construct a glucose biosensor through self-assembly. The resulting modified electrode surfaces are characterized with both AFM and cyclic voltammetry. Effects of chitosan and HAuCl 4 concentration in the mixture together with the deposition time and the applied voltage on the amperometric response of the biosensor are also investigated. The linear range of the glucose biosensor is from 5.0 × 10 − 5 ∼ 1.30 × 10 − 3  M with a Michaelis–Menten constant of 3.5 mM and a detection limit of about 13 μM.