The anti-inflammatory activity of peptides from simulated gastrointestinal digestion of preserved egg white in DSS-induced mouse colitis

• Published in: Food & function Vol. 9; no. 12; pp. 6444 - 6454
• Main Authors: Zhang, Mengya, Zhao, Yan, Wu, Na, Yao, Yao, Xu, Mingsheng, Du, Huaying, Tu, Yonggang
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 13.12.2018
• Subjects: Albumen; Anti-inflammatory agents; Colitis; Colon; Computer simulation; Cytokines; Dextran; Digestion; Digestive system; Gastrointestinal tract; Gene expression; Hypertrophy; IL-1β; Inflammatory bowel disease; Inflammatory bowel diseases; Interleukin 17; Interleukin 6; Intestine; Medical treatment; Mice; Monocyte chemoattractant protein 1; Peptides; Rodents; Sodium; Sodium sulfate; Spleen; Tumor necrosis factor-α; Weight loss; γ-Interferon
• ISSN: 2042-6496; 2042-650X
• DOI: 10.1039/c8fo01939h

Peptides DEDTQAMPFR (DR-10), MLGATSL (ML-7), SLSFASR (SR-7), and MSYSAGF (MF-7) derived from simulated gastrointestinal digestion of preserved egg white (SGD-PEW) exerted anti-inflammatory effects on Caco-2 cells. Here, we aimed to evaluate the anti-inflammatory effects of these peptides derived from SGD-PEW in a mouse model of dextran sodium sulfate (DSS)-induced colitis. The results showed that DR-10, ML-7, SR-7 and MF-7 significantly ameliorated the clinical symptoms of DSS-induced mice colitis, such as weight loss, disease activity index (DAI), colon shortening, spleen hypertrophy and histological scores. Treatment with DR-10, ML-7, SR-7 and MF-7 also significantly inhibited the local secretion of pro-inflammatory cytokines TNF-α and IL-6 and markedly decreased the gene expression of pro-inflammatory cytokines, including TNF-α, IL-6, IL-17, IL-1β, IFN-γ and MCP-1, in DSS-induced mice colitis. Overall, MF-7 showed the best effect of alleviating DSS-induced colitis among the four peptides. These results suggested that MF-7, DR-10, ML-7 and SR-7 may be a potential promising candidate for the treatment of inflammatory bowel disease (IBD). Peptides DEDTQAMPFR (DR-10), MLGATSL (ML-7), SLSFASR (SR-7), and MSYSAGF (MF-7) derived from simulated gastrointestinal digestion of preserved egg white (SGD-PEW) exerted anti-inflammatory effects on DSS-induced mouse colitis.