Affinity informs environmental cooperation between ammonia-oxidizing archaea (AOA) and anaerobic ammonia-oxidizing (Anammox) bacteria

• Published in: The ISME Journal Vol. 13; no. 8; pp. 1997 - 2004
• Main Authors: Straka, Levi L., Meinhardt, Kelley A., Bollmann, Annette, Stahl, David A., Winkler, Mari-K. H.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.08.2019; Nature Publishing Group
• Subjects: 631/158/1745; 631/158/855; Affinity; Ammonia; Ammonia - chemistry; Ammonia - metabolism; Ammonia-oxidizing bacteria; Anaerobiosis; Archaea; Archaea - chemistry; Archaea - classification; Archaea - isolation & purification; Archaea - metabolism; Bacteria; Bacteria - chemistry; Bacteria - classification; Bacteria - isolation & purification; Bacteria - metabolism; Biofilms; Biomedical and Life Sciences; Cooperation; Ecology; Evolutionary Biology; Kinetics; Lakes; Lakes - microbiology; Life Sciences; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Nitrogen dioxide; Oxidation; Oxidation-Reduction; Oxygen; Oxygen - metabolism; Oxygen enrichment; Phylogeny; Soil - chemistry; Soil Microbiology
• ISSN: 1751-7362; 1751-7370
• DOI: 10.1038/s41396-019-0408-x

Anaerobic ammonia-oxidizing (Anammox) bacteria (AnAOB) rely on nitrite supplied by ammonia-oxidizing bacteria (AOB) and archaea (AOA). Affinities for ammonia and oxygen play a crucial role in AOA/AOB competition and their association with AnAOB. In this work we measured the affinity constants for ammonia and oxygen (half-saturation; k m ) of two freshwater AOA enrichments, an AOA soil isolate ( N. viennensis ), and a freshwater AnAOB enrichment. The AOA enrichments had similar kinetics ( μ max ≈ 0.36 d −1 , k m,NH4 ≈ 0.78 µM, and k m,O2 ≈ 2.9 µM), whereas N. viennensis had similar k m values but lower μ max (0.23 d −1 ). In agreement with the current paradigm, these AOA strains showed a higher affinity for ammonia (lower k m,NH4 ; 0.34–1.27 µM) than published AOB measurements (>20 µM). The slower growing AnAOB (μ max ≈ 0.16 d −1 ) had much higher k m values ( k m,NH4 ≈ 132 µM, k m,NO2 ≈ 48 µM) and were inhibited by oxygen at low levels (half-oxygen inhibition; k i,O2 ≈ 0.092 µM). The higher affinity of AOA for ammonia relative to AnAOB, suggests AOA/AnAOB cooperation is only possible where AOA do not outcompete AnAOB for ammonia. Using a biofilm model, we show that environments of ammonia/oxygen counter diffusion, such as stratified lakes, favors this cooperation.