Toward point-of-care assessment of patient response: a portable tool for rapidly assessing cancer drug efficacy using multifrequency impedance cytometry and supervised machine learning

• Published in: Microsystems & nanoengineering Vol. 5; no. 1; pp. 1 - 11
• Main Authors: Ahuja, Karan, Rather, Gulam M., Lin, Zhongtian, Sui, Jianye, Xie, Pengfei, Le, Tuan, Bertino, Joseph R., Javanmard, Mehdi
• Format: Journal Article
• Language: English
• Published: London Springer Nature B.V 15.07.2019; Nature Publishing Group UK
• Subjects: Antibodies; Antineoplastic drugs; Artificial intelligence; Cancer; Cell surface; Cell viability; Cytometry; Labeling; Learning algorithms; Machine learning; Phase transitions; Sensitivity analysis; Spectroscopy; Spectrum analysis; Staining; Support vector machines; Surface markers; Tumor cells; Tumors
• ISSN: 2055-7434; 2096-1030; 2055-7434
• DOI: 10.1038/s41378-019-0073-2

Abstract We present a novel method to rapidly assess drug efficacy in targeted cancer therapy, where antineoplastic agents are conjugated to antibodies targeting surface markers on tumor cells. We have fabricated and characterized a device capable of rapidly assessing tumor cell sensitivity to drugs using multifrequency impedance spectroscopy in combination with supervised machine learning for enhanced classification accuracy. Currently commercially available devices for the automated analysis of cell viability are based on staining, which fundamentally limits the subsequent characterization of these cells as well as downstream molecular analysis. Our approach requires as little as 20 μL of volume and avoids staining allowing for further downstream molecular analysis. To the best of our knowledge, this manuscript presents the first comprehensive attempt to using high-dimensional data and supervised machine learning, particularly phase change spectra obtained from multi-frequency impedance cytometry as features for the support vector machine classifier, to assess viability of cells without staining or labelling.