Complete nucleotide sequence of ovine β-casein cDNA: inter-species comparison

• Published in: Biochimie Vol. 71; no. 7; pp. 827 - 832
• Main Authors: Provot, Christian, Persuy, Marie-Annick, Mercier, Jean-Claude
• Format: Journal Article
• Language: English
• Published: Paris Elsevier Masson SAS 01.07.1989; Elsevier
• Subjects: Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Animals; Base Sequence; Biochemistry, Molecular Biology; Biological and medical sciences; Biological Evolution; CASEIN; CASEINA; CASEINE; Caseins - genetics; Cattle; cDNA sequence; CHEMICAL STRUCTURE; DNA - genetics; ESTRUCTURA QUIMICA; EWE MILK; Exons; Fundamental and applied biological sciences. Psychology; Genes; Holoproteins; Introns; LAIT DE BREBIS; LECHE DE OVEJA; Life Sciences; Mice; Molecular Sequence Data; Mutation; NUCLEOTIDE; NUCLEOTIDES; NUCLEOTIDOS; Other proteins; ovine β-casein; Plasmids; Proteins; Rabbits; Rats; Restriction Mapping; RNA, Messenger - genetics; Sequence Homology, Nucleic Acid; Sheep - genetics; STRUCTURE CHIMIQUE; cDNA sequence; ovine β-casein; In vitro transcription; Recombination; Casein; Complementary DNA; Nucleotide sequence; Phosphoproteins; Interspecific comparison; Restriction map; Enzymatic digestion; Molecular cloning; Nucleic acid; Aminoacid sequence; RAT
• ISSN: 0300-9084; 1638-6183
• DOI: 10.1016/0300-9084(89)90046-1

The complete nucleotide sequence of ovine β-casein mRNA has been determined by sequencing, according to Sanger-Messing, both a recombinant clone isolated from a mammary cDNA pUC 18 library and a single-stranded cDNA generated by reverse transcription from a synthetic 17-mer primer complementary to the 5′ part of the mRNA coding frame. The 1088 nucleotide long β-casein mRNA, excluding the poly(A) tail, contains a coding frame of 669 nucleotides including the stop codon, flanked by 60 adn 359 nucleotides in the 5′ and 3′ untranslated regions, respectively. It arises from the splicing of 9 exons as deduced from gene sequence data. The deduced amino acid sequence differs at 3 positions from that previously determined by direct sequencing of mature β-casein. Comparison of the ovine, bovine, rat, mouse, and rabbit β-casein mRNA sequences shows a higher homology in the 3′ and 5′ untranslated regions. The most conserved regions in the open reading frame are essentially those encoding the signal peptide and the major phosphorylation site.