Studies on Chemical Composition of Pueraria lobata and Its Anti-Tumor Mechanism

Fourteen compounds were isolated from Pueraria lobata (Willd.) Ohwi by column chromatography and preparative thin-layer chromatography; the structures were identified by spectroscopic analysis and compared with data reported in the literature. Seven compounds were isolated and identified from Puerar...

Full description

Saved in:
Bibliographic Details
Published inMolecules (Basel, Switzerland) Vol. 27; no. 21; p. 7253
Main Authors Fang, Xiaoxue, Zhang, Yegang, Cao, Yiming, Shan, Mengyao, Song, Dimeng, Ye, Chao, Zhu, Difu
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 26.10.2022
MDPI
Subjects
Aldehydes
Apoptosis
Breast cancer
Cancer therapies
cell cycle
Cell proliferation
Chemical composition
Cholecystokinin
Colorectal cancer
Column chromatography
Drug resistance
Genistein
Hyperlipidemia
Kinases
Linoleic acid
Medical screening
network pharmacology
Nitric oxide
Ovarian cancer
Pharmacology
Pueraria lobata
sandwicensin
Syringaldehyde
Thin layer chromatography
TOR protein
Tumor necrosis factor-TNF
Tumors
Online AccessGet full text

Cover

More Information
Summary:Fourteen compounds were isolated from Pueraria lobata (Willd.) Ohwi by column chromatography and preparative thin-layer chromatography; the structures were identified by spectroscopic analysis and compared with data reported in the literature. Seven compounds were isolated and identified from Pueraria lobata for the first time: Linoleic acid, Sandwicensin, Isovanillin, Ethyl ferulate, Haginin A, Isopterofuran, 3′.7-Dihydroxyisoflavan. The other 10 compounds were structurally identified as follows: Lupenone, Lupeol, β-sitosterol, Genistein, Medicarpin, Coniferyl Aldehyde, Syringaldehyde. All compounds were evaluated for their ability to inhibit SW480 and SW620 cells using the CCK-8 method; compound 5 (Sandwicensin) had the best activity, and compounds 6, 9, 11 and 12 exhibited moderate inhibitory activity. In addition, the targets and signaling pathways of Sandwicensin treatment for CRC were mined using network pharmacology, and MAPK3, MTOR, CCND1 and CDK4 were found to be closely associated with Sandwicensin treatment for CRC; the GO and KEGG analysis showed that Sandwicensin may directly regulate the cycle, proliferation and apoptosis of CRC cells through cancer-related pathways.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
These authors contributed equally to this work.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27217253