Molecular Mechanism of Substrate Specificity for Heparan Sulfate 2-O-Sulfotransferase

• Published in: The Journal of biological chemistry Vol. 289; no. 19; pp. 13407 - 13418
• Main Authors: Liu, Chunhui, Sheng, Juzheng, Krahn, Juno M., Perera, Lalith, Xu, Yongmei, Hsieh, Po-Hung, Dou, Wenfang, Liu, Jian, Pedersen, Lars C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 09.05.2014; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Catalytic Domain; Chickens; Crystallography, X-Ray; Glycobiology and Extracellular Matrices; Heparan Sulfate; Heparin; Heparin-binding Protein; Oligosaccharide; Structure-Activity Relationship; Substrate Specificity - physiology; Sulfotransferase; Sulfotransferases - chemistry; Sulfotransferases - genetics; Sulfotransferases - metabolism; Heparan Sulfate; Heparin; Heparin-binding Protein; Oligosaccharide; Sulfotransferase
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M113.530535

Heparan sulfate (HS) is an abundant polysaccharide in the animal kingdom with essential physiological functions. HS is composed of sulfated saccharides that are biosynthesized through a complex pathway involving multiple enzymes. In vivo regulation of this process remains unclear. HS 2-O-sulfotransferase (2OST) is a key enzyme in this pathway. Here, we report the crystal structure of the ternary complex of 2OST, 3′-phosphoadenosine 5′-phosphate, and a heptasaccharide substrate. Utilizing site-directed mutagenesis and specific oligosaccharide substrate sequences, we probed the molecular basis of specificity and 2OST position in the ordered HS biosynthesis pathway. These studies revealed that Arg-80, Lys-350, and Arg-190 of 2OST interact with the N-sulfo groups near the modification site, consistent with the dependence of 2OST on N-sulfation. In contrast, 6-O-sulfo groups on HS are likely excluded by steric and electrostatic repulsion within the active site supporting the hypothesis that 2-O-sulfation occurs prior to 6-O-sulfation. Our results provide the structural evidence for understanding the sequence of enzymatic events in this pathway. Sulfotransferases with distinct specificities act in sequence in the heparan sulfate biosynthetic pathway. The crystal structure of 2-O-sulfotransferase with bound substrate reveals its requirements for substrate recognition. The 2-O-sulfotransferase recognizes N-sulfate but excludes 6-O-sulfate on substrates. The results advance the understanding of cellular control for the biosynthesis of heparan sulfate.