Increased oxidative stress contributes to impaired peripheral CD56dimCD57+ NK cells from patients with systemic lupus erythematosus

• Published in: Arthritis research & therapy Vol. 24; no. 1; pp. 1 - 48
• Main Authors: Lu, Zhimin, Tian, Yao, Bai, Ziran, Liu, Jiaqing, Zhang, Yan, Qi, Jingjing, Jin, Minli, Zhu, Jie, Li, Xia
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 16.02.2022; BioMed Central; BMC
• Subjects: Age; Analysis; Antibodies; Apoptosis; Arthritis; Blood; Care and treatment; Cytokines; Cytotoxicity; Diagnosis; Disease; Flow cytometry; Gender; Gene expression; Health aspects; Hemoglobin; Hospitals; Immune response; Immunoglobulins; Killer cells; Lupus; Lymphocytes; Natural killer cell; Oxidative stress; Proteins; Reactive oxygen species; Risk factors; Software; Subset; Systemic lupus erythematosus; China
• ISSN: 1478-6362; 1478-6354; 1478-6362
• DOI: 10.1186/s13075-022-02731-y

Abstract Background Systemic lupus erythematosus (SLE) is characterized by loss of immune tolerance and imbalance of immune cell subsets. Natural killer (NK) cells contribute to regulate both the innate and adaptive immune response. In this study, we aimed to detect alterations of peripheral NK cells and explore intrinsic mechanisms involving in NK cell abnormality in SLE. Methods Blood samples from healthy controls (HCs) and patients with SLE and rheumatoid arthritis (RA) were collected. The NK count, NK subsets (CD56 bright , CD56 dim CD57 − , and CD56 dim CD57 + ), phenotypes, and apoptosis were evaluated with flow cytometer. Mitochondrial reactive oxygen species (mtROS) and total ROS levels were detected with MitoSOX Red and DCFH-DA staining respectively. Published data (GSE63829 and GSE23695) from Gene Expression Omnibus (GEO) was analyzed by Gene Set Enrichment Analysis (GSEA). Results Total peripheral NK count was down-regulated in untreated SLE patients in comparison to that in untreated RA patients and HCs. SLE patients exhibited a selective reduction in peripheral CD56 dim CD57 + NK cell proportion, which was negatively associated with disease activity and positively correlated with levels of complement(C)3 and C4. Compared with HCs, peripheral CD56 dim CD57 + NK cells from SLE patients exhibited altered phenotypes, increased endogenous apoptosis and higher levels of mtROS and ROS. In addition, when treated with hydrogen peroxide (H 2 O 2 ), peripheral CD56 dim CD57 + NK cell subset was more prone to undergo apoptosis than CD56 dim CD57 − NK cells. Furthermore, this NK cell subset from SLE patients exhibited impaired cytotoxicity in response to activated CD4 + T cells in vitro. Conclusion Our study demonstrated a selective loss of mature CD56 dim CD57 + NK cell subset in SLE patients, which may caused by preferential apoptosis of this subset under increased oxidative stress in SLE. The attenuated in vitro cytotoxicity of CD56 dim CD57 + NK cells may contribute to the impaired ability of eliminating pathogenic CD4 + T cells in SLE.