Determination of antioxidant activity of Hibiscus sabdariffa and Croton caudatus in Saccharomyces cerevisiae model system

• Published in: Journal of food science and technology Vol. 54; no. 9; pp. 2728 - 2736
• Main Authors: Subhaswaraj, Pattnaik, Sowmya, M, Bhavana, V, Dyavaiah, Madhu, Siddhardha, Busi
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.08.2017; Springer Nature B.V
• Subjects: Antioxidants; Ascorbic acid; Baking yeast; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Clonal deletion; Flowers & plants; Fluorescence; Food Science; Free radicals; Herbal medicine; Hibiscus sabdariffa; Hydrogen peroxide; In vitro methods and tests; In vivo methods and tests; Leaves; Medicinal plants; Nutrition; Original; Original Article; Plant extracts; Saccharomyces cerevisiae; Scavenging; Studies; Superoxide dismutase; Yeast; Yeasts; Dichlorofluorescin; Reactive oxygen species; DPPH; Antioxidant activity; Saccharomyces cerevisiae
• ISSN: 0022-1155; 0975-8402
• DOI: 10.1007/s13197-017-2709-2

From ancient times, plants and plant derived products are exploited as a prominent source of folkloric medicines with tremendous therapeutic potential for an array of health disorders. In the present study, ethanolic leaf extract of Hibiscus sabdariffa and Croton caudatus were evaluated for free radical scavenging activity in Saccharomyces cerevisiae model system. H. sabdariffa and C. caudatus showed tremendous DPPH free radical scavenging potential with an IC 50 value of 184.88 and 305.39 µg/mL respectively at a concentration of 500 µg/mL. The ethanolic leaf extract of H. sabdariffa and C. caudatus also showed significant hydoxyl radical scavenging and total antioxidant activity. Ascorbic acid was used as positive control. The in vitro antioxidant activity was further supported by in vivo studies using radical scavenging mechanism in S. cerevisiae wild type and its isogenic deletion strains sod1∆ and tsa1∆ . The mutant yeast cells substantially scavenged the stress generated by H 2 O 2 when supplemented with ethanolic leaf extract of H. sabdariffa and C. caudatus as evident from spot assays followed by fluorescence assay (DCF-DA) using fluorescence microscopic and intensity studies. H. sabdariffa and C.caudatus significantly neutralize the ROS level in yeast mutants with concomitant decrease in fluorescence intensity as compared to the untreated yeast cells. The results suggested the efficacy of H. sabdariffa and C. caudatus as potent antioxidants in yeast system and thus their futuristic applications in therapeutics.