Cardioprotective Effect of Modified Peroxiredoxins in Retrograde Perfusion of Isolated Rat Heart under Conditions of Oxidative Stress

• Published in: Bulletin of experimental biology and medicine Vol. 160; no. 5; pp. 639 - 642
• Main Authors: Karaduleva, E. V., Mubarakshina, E. K., Sharapov, M. G., Volkova, A. E., Pimenov, O. Yu, Ravin, V. K., Kokoz, Yu. M., Novoselov, V. I.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2016; Springer; Springer Nature B.V
• Subjects: Animals; Antioxidants; Antioxidants - therapeutic use; Biomedical and Life Sciences; Biomedicine; Cardiotonic Agents - therapeutic use; Cell Biology; Enzymes; Heart - physiopathology; Heart beat; Heart Rate - drug effects; Hydrogen Peroxide - adverse effects; Internal Medicine; Laboratory Medicine; Male; Myocardial Contraction - drug effects; Myocardial Reperfusion Injury - drug therapy; Myocardial Reperfusion Injury - prevention & control; Oxidative stress; Oxidative Stress - drug effects; Pathology; Perfusion; Peroxidase - metabolism; Peroxiredoxins - therapeutic use; Rats; Rats, Wistar; Superoxide; Superoxide Dismutase - metabolism; retrograde perfusion of the heart; peroxiredoxins; oxidative stress
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-016-3237-1

Antioxidant properties of recombinant peroxiredoxin-6 and chimeric protein PSH combining peroxidase and superoxide dismutase activities were studied on the model of retrograde perfusion of isolated rat heart under conditions of H 2 O 2 -induced oxidative stress. The exogenous antioxidant proteins exhibited cardioprotective properties manifested in heart rate normalization, maintenance of contractile activity of the myocardium, and prevention of H 2 O 2 -induced LPO in oxidative stress. Localization of peroxiredoxin-6 and PSH in the cardiac tissue was determined and myocardial structures most effectively protected by the antioxidant enzymes from ischemia/reperfusion-induced damages were identified. The results suggest that modified peroxiredoxins are promising components of perfusion media for preservation of isolated organs.