Self-cloning of the Catalase Gene in Environmental Isolates Improves Their Colony-forming Abilities on Agar Media

Hydrogen peroxide (H2O2) inhibits microbial growth at a specific concentration. However, we previously isolated two environmental bacterial strains that exhibited sensitivity to a lower H2O2 concentration in agar plates. Putative catalase genes, which degrade H2O2, were detected in their genomes. We...

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Bibliographic Details
Published inMicrobes and Environments Vol. 38; no. 2; p. ME23006
Main Authors Watanabe, Motoyuki, Igarashi, Kensuke, Kato, Souichiro, Kamagata, Yoichi, Kitagawa, Wataru
Format Journal Article
LanguageEnglish
Published Japan Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2023
Subjects
Agar
agar plate
catalase
Catalase - genetics
Cloning, Molecular
colony formation
Culture Media
hydrogen peroxide
Hydrogen Peroxide - pharmacology
self-cloning
Short Communication
agar plate
colony formation
self-cloning
catalase
hydrogen peroxide
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Summary:Hydrogen peroxide (H2O2) inhibits microbial growth at a specific concentration. However, we previously isolated two environmental bacterial strains that exhibited sensitivity to a lower H2O2 concentration in agar plates. Putative catalase genes, which degrade H2O2, were detected in their genomes. We herein elucidated the characteristics of these putative genes and their products using a self-cloning technique. The products of the cloned genes were identified as functional catalases. The up-regulation of their expression increased the colony-forming ability of host cells under H2O2 pressure. The present results demonstrated high sensitivity to H2O2 even in microbes possessing functional catalase genes.
ISSN:1342-6311
1347-4405
DOI:10.1264/jsme2.ME23006