The protective effect of Ganoderma lucidum on testicular torsion/detorsion-induced ischemia-reperfusion (I/R) injury

• Published in: Acta cirurgica brasileira Vol. 35; no. 1; p. e202000103
• Main Authors: Doğan, Gül, İpek, Hülya
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01.01.2020
• Subjects: Animals; Antioxidants - pharmacology; Antioxidants - therapeutic use; Catalase - metabolism; Drug Evaluation, Preclinical; Lymphoma, B-Cell; Male; Malondialdehyde - metabolism; Random Allocation; Rats; Rats, Wistar; Reishi; Reishi - chemistry; Reperfusion Injury - etiology; Reperfusion Injury - prevention & control; Spermatic Cord Torsion; Spermatic Cord Torsion - complications; Spermatic Cord Torsion - metabolism; Superoxide Dismutase - metabolism; Testis; Testis - blood supply; Testis - drug effects; Testis - pathology; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factor A - metabolism
• ISSN: 0102-8650; 1678-2674
• DOI: 10.1590/s0102-865020200010000003

To investigate the protective effect of Ganoderma lucidum on testicular torsion/detorsion (T/D)-induced ischemia-reperfusion (I/R) injury. Thirty male Wistar albino rats were randomly categorized into 3 groups: Group 1: sham, Group 2 ( T/D): 2,5 hours of ischemia and 7 days of reperfusion, Group 3 (T/D+ G. lucidum ): 2,5 hours of ischemia and 7 days of reperfusion and 7 days of 20 mg/kg via gastric gavage G. lucidum polysaccharides per day. Biochemical assays of Malondialdehyde (MDA), superoxide dismutase (SOD), Catalase (CAT), Glutathione (GSH) levels , histopathology and expression levels of VEGF and Bcl-2 with immunohistochemical methods were examined in testicular tissue. G. lucidum treatment was found to have prevented the T/D-induced I/R injury by decreasing MDA levels of the testis. SOD, CAT and GSH activities were decreased in group 2, while they were increased in group 3 (p<0.001) and significant improvement in the tube diameter was observed in group 3. Bcl-2-positive germinal cells were lowered in group 3 compared to the group 2. VEGF expression showed an increase in group 2, whereas it decreased in group 3. The antioxidant G. lucidum is thought to induce angiogenesis by reducing the apoptotic effect in testicular torsion-detorsion.