Hydrogen/deuterium exchange behavior in tetragonal hen egg-white lysozyme crystals affected by solution state

Neutron diffraction studies of hydrogen/deuterium-exchanged hen egg-white lysozyme were performed by a joint X-ray and neutron refinement to elucidate the hydrogen/deuterium exchange behavior. Large crystals for neutron work, consisting of molecules that were exchanged before crystallization, were o...

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Bibliographic Details
Published inJournal of applied crystallography Vol. 53; no. 3; pp. 837 - 840
Main Authors Kita, Akiko, Morimoto, Yukio
Format Journal Article
LanguageEnglish
Published Oxford Blackwell Publishing Ltd 01.06.2020
International Union of Crystallography
Subjects
Crystallization
Crystallography
Crystals
Deuteration
Deuterium
Exchanging
Hydrogen
Lysozyme
Neutron diffraction
Neutrons
NMR
Nuclear magnetic resonance
Reagents
Short Communications
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Summary:Neutron diffraction studies of hydrogen/deuterium-exchanged hen egg-white lysozyme were performed by a joint X-ray and neutron refinement to elucidate the hydrogen/deuterium exchange behavior. Large crystals for neutron work, consisting of molecules that were exchanged before crystallization, were obtained by repeatedly adding protein solution to the crystal batch using deuterated precipitant reagent. There are differences in hydrogen/deuterium exchange behavior compared with previous crystallographic or NMR studies, which could be due to intermolecular interactions in the crystal or to different lengths of exchange period.
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ISSN:1600-5767
0021-8898
1600-5767
DOI:10.1107/S1600576720005488